Listen In - Bitesize Bio Webinar Audios – Details, episodes & analysis

In this episode, we explore the cutting-edge techniques revolutionizing fluorescence microscopy. Traditional methods are limited to detecting three to four targets due to the spectral overlap of fluorophores.

But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluorescence lifetimes to distinguish between similar dyes, allowing for more complex imaging.

Hear how researchers combined lifetime multiplexing with spectral detection to analyze multiple targets simultaneously. Discover the exciting advancements made by characterizing 18 synthetic fluorophores and developing unique self-labeling protein tags.

These breakthroughs enable the imaging of up to eight targets in four spectral channels, providing unprecedented detail in cellular studies.

Watch the full presentation here: https://microscopyfocus.com/live-cell-fluorescence-lifetime-multiplexing-using-organic-fluorophores/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Stress is a universal challenge, and scientists face unique pressures such as impostor syndrome, the demand for positive results, and career uncertainty. These stressors can be overwhelming and often feel beyond our control.

In this episode of Listen In, we explore the various stresses that scientists encounter in their daily lab life and discuss effective strategies to manage them. Learn how to prevent burnout, anxiety, and depression by addressing issues like perfectionism and impostor syndrome. Our expert shares personal insights and practical advice on cultivating a resilient and healthier growth mindset.

Hit play to discover that you're not alone in feeling overwhelmed and that with the right mindset, you can navigate the pressures of a scientific career more effectively.

Watch the full presentation here: https://events.bitesizebio.com/stress-in-the-lab-common-pitfalls-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Despite the growing number of new technologies and automated instruments, routine liquid handling is still a manual process in many labs. The trend towards automation is gaining importance, not only with complex applications, such as NGS library preparation but also with simple yet repetitive liquid handling steps where automated pipetting makes sense.

A variety of automated liquid handling systems—suiting all budgets and workloads—are now available. These systems are designed to improve consistency and efficiency by eliminating both person-to-person and day-to-day variability.

But how to get started with automation? When does it make sense to automate workflows and methods, and isn't it super complicated to implement an automated liquid handler in a lab?

Get answers to these questions in this episode of Listen In!

Watch the full presentation here: https://events.bitesizebio.com/automate-your-pipetting-unleash/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Expression and Purification of an Engineered, E. coli-expressed Leishmania donovani Nucleoside Hydrolase with Immunogenic Properties
Potential recombinant protein vaccine candidates must meet several criteria:

They must be expressed at sufficiently high levels in the organism of choice
They must be purified to high purity from the expression system in an immunogenic form
They must induce potent immune responses
Dr. Patrick McAtee will take you through these vaccine development steps using the nucleoside hydrolase antigen from Leishmania donovani as an example. He will demonstrate how his lab cloned and expressed the full-length, 36-dKa protein. He will discuss purification of the protein to >99% purity using anion exchange and gel filtration chromatography. He will also talk about the steps taken to ensure protein integrity and enzymatic activity using lithium dodecyl sulfate polyacrylamide gel electrophoresis (LDS-PAGE), mass spectrometry (MS), and enzymatic assays.

Dr. McAtee will then take you through in vivo testing of the vaccine candidate including analyzing antibody levels from mice immunized with the protein alone or in a stable emulsion with glucopyranosyl lipid adjuvant (GLA-SE). He will describe characterization of the type of cellular immune response induced by the protein. Finally, he will demonstrate protective efficacy in mice challenged with Leishmania mexicana.

For more information visit: https://bitesizebio.com/webinar/development-of-a-potential-recombinant-protein-vaccine-in-e-coli/

In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research.

The main points in the webinar will include:

How to design guide RNAs using online tools specific to the genome and application of interest.
Tips and practical advice to assist you in choosing and optimizing a CRISPR/Cas9 delivery system.
How you can create precise mutations using homology-directed repair, including template design and cleavage site
An Essential Guide to CRISPR/Cas9 Editing Efficiency
While CRISPR/Cas9 editing is utilized in a wide variety of cell types, editing efficiency continues to pose a challenge to researchers. Join Dr. Allison Mayle, as she shares best practices for increasing CRISPR/Cas9 editing efficiency. Viewers will discover online tools to aid in CRISPR/Cas9 design and delivery and tips for optimizing your CRISPR/Cas9 experiments.

In this webinar, Dr. Mayle will review the factors influencing genome editing, including target sequence selection and CRISPR delivery methods. A comparison of plasmid and viral vector delivery will be provided, as well as an introduction to DNA-free CRISPR/Cas9 ribonucleoprotein reagents. Additionally, Dr. Mayle will cover best practices for CRISPR knock-in mutagenesis via homology-directed repair (HDR) and applications available from new Cas9 protein variants.

For more information visit: https://bitesizebio.com/webinar/guide-crisprcas9-delivery-maximize-editing-efficiency/

Join Dr. Karen O’Hanlon Cohrt for a practical tour of multiplex PCR technology, where you will learn the following and much more:

Principle behind multiplex PCR technology
Popular applications of this technology
How to set up this reaction
Advantages and disadvantages
Multiplex PCR Can Benefit Your Research
Dr O’Hanlon Cohrt will discuss the history of multiplex PCR, how the technique works, and how to set up a reaction. She will also provide advice for optimization of primers and how to detect your targets.

Multiplex PCR technology simultaneously detects multiple nucleic acid targets in a single reaction. This method is a straightforward and efficient solution for bypassing challenges associated with limited template material. As an added bonus, this technology is cost effective because fewer reactions are needed to detect disparate targets.

Karen will show you the real beauty of multiplex PCR— how you can use very little template or sample in each experiment. She will also share the applications of this technology, which include forensic science, pathogen identification, and genetic testing—especially in cancer.

For more information visit: https://bitesizebio.com/webinar/multiplex-pcr-technology/

The rise of open access is changing how research is communicated. In this webinar, we’ll celebrate Open Access Week 2016 by taking a closer look at how open access affects how researchers write and publish their results. Specifically, we will:

Define open access and Creative Commons licensing and dive into the numbers about open access (journals, articles, fees)
Discuss the pros and cons of open access and dispel some myths
Describe how funder and government mandates will affect the open access movement and individual researchers

For more information visit: https://bitesizebio.com/webinar/changing-landscape-open-access-publishing/

In this webinar, you will learn about color reproduction in microscopy images—and how to fix it if your color reproduction goes awry.

The main points we will cover are:

The reasons that could color reproduction go wrong in your microscopy images
How to correct your microscopy images with inappropriate color reproduction
How to be ethical with the color in your microscopy images
Advice on Color in Microscopy Images
Mark Clymer, an expert in microscopy imaging, will guide you through the complicated area of color reproduction in microscopy. He will offer advice and tips on how to get the most from your microscopy camera.

Color imaging is everywhere today and is often taken for granted. Images are instantly captured on cell phones, tablets, web cams, and microscopes. Pictures are shared with friends and colleagues even faster. We thrive on instant gratification. For some of us, our images are simply a snapshot in time—a selfie in front of a landmark, an amazing meal presentation, a pic showing viable cells in culture. But for others, images need to be masterpieces, expressing not just artistry but scientific discovery.

Regardless of your philosophy, we are at the mercy of the technology: cameras AND software. So as scientists, how do we ensure that we capture and communicate images that are worthy of our research? In this webinar, you’ll learn about how color in images goes awry, and what you can do about it. And there’s a twist at the end about an often overlooked villain in this whole scheme.

About the Presenter:
Mark Clymer is a veteran of the microscopy industry, establishing his fundamental skills in biotech and the drug discovery labs at Sanofi, and honing those skills as product manager at Olympus, managing the core microscope product line in the Americas. Along the way, Mark attended microscopy-based courses at the Marine Biological Laboratory in Woods Hole, MA, and now shares his expertise with students during the Immunohistochemistry & Microscopy course held each March at the MBL.

Mark offers consulting services to microscopy and biomedicine-based companies, and is the former Director of Marketing for the Datacolor Scientific division of Datacolor Inc., the Swiss-headquartered market leader in color management for industry and photography. He is the author of numerous articles and blogs on microscopy and color management. You may have seen some of his posts to microscopy and imaging-based groups on LinkedIn. Mark is also an independent microscopy and spectrophotometry sales specialist for Laxco Inc.

Mark invites you to look him up on LinkedIn

For more information visit: https://bitesizebio.com/webinar/color-image-quality-control-microscopy/

Incorporating New Dyes to Simplify Panel Design: Accelerating Reagent Availability

In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components.

We will cover:

How quality custom reagents in small sizes will make your panel design easier
How you can receive these reagents fast and without traditional wait times for manufacturing
How you can be confident that quality control is maintained for these custom reagents
Incorporating New Dyes to Simplify Panel Design
Dr. Jorg Ruhrer, Director of Research Reagents at BD Bioscience will take through how to overcome a major limitation in multicolor panel design for flow cytometry experiments—limited antibody-fluorochrome combinations. Join him in this webinar as he shows you how custom reagents provide increased options in your multicolor panel design.

Flow cytometry continues to be a critical tool for the analysis, characterization, and isolation of single cells from a heterogeneous cell population. The rapid introduction of dyes based on Sirigen technology has dramatically increased the total number of parameters that can be analyzed simultaneously from a single sample. Using these dyes coupled with recent advancements in flow cytometers, it is now possible to probe more than 28 different fluorescence parameters simultaneously, allowing for a much deeper biological understanding of a sample.

However, in spite of these advancements, one of the major limitations to designing optimal multicolor panels is the unavailability of many reagents and specificities in these new fluorochromes. To address this need, BD Biosciences has launched a new product line that will give scientists immediate access to hundreds and eventually thousands of new antibody/bright dye combinations. These new combinations will give the flexibility that you need to design a multicolor panel without compromising on results.

Jorg will discuss how these quality reagents will enable easier panel design, resulting in robust high content panels that will generate quality data to drive ever deeper scientific discoveries.

For more information visit: https://bitesizebio.com/webinar/incorporating-new-dyes-simplify-panel-design/

In this webinar, you will learn about the process for genotyping cells—particularly tumor cells—and practical advice for analyzing the resulting data.

The main points that we will cover are:

Practical advice and methods for sample collection and preparation for your genotyping experiments
An overview of the available data analysis methods for your genotyping results
Examples of pathway analysis methods for determining potential mechanisms within your system
Advice, tips, and applications for your genotyping experiments
Dr. Ania Wronski, a breast cancer researcher from Tufts University, will guide you through the process of investigating the genotype of tumor cells. Join her in this webinar as she takes you from sample collection to data analysis.

The American Cancer Society predicts that 1.7 million new cancer cases will be diagnosed and almost 600,000 cancer deaths will occur this year in the United States alone. These staggering numbers prompted the creation of the National Cancer Moonshot initiative in the US and contributed to the prominent position of cancer research in the European Union’s Horizon 2020 project.

Recent evidence suggests that cells within a particular tumor are not all genetically identical. Most tumors exhibit some degree of cellular heterogeneity. While most cells within the tumor possess one or more dominant mutations, sometimes there is a subpopulation with different ones. Researchers hypothesize that these subpopulations might be responsible for resistance to therapeutic agents. Discovering the genotype of tumor cells might yield new therapeutic targets to aid in the fight against multidrug resistance of cancer.

Ania will use her own research as an example and give you a broad strokes overview of the steps necessary to interrogate the genotype of tumors. She will lead you through the collection and preparation of samples and discuss available data analysis methods. Finally, she will share examples of pathway analysis that can be used to discover potential mechanisms.

For more information visit: https://bitesizebio.com/webinar/practical-advice-genotyping-cancer-cells/