In this Micro Minutes episode, Luis breaks down classic microbiology traits that usually hold true, but not always.  From indole-negative E. coli to non-swarming Proteus and oxidase-negative Pseudomonas, this quick episode highlights real-world exceptions that can catch techs and students off guard.

Learn how to spot:

• Indole-negative E. coli (98% rule + inactive biotypes)

• Lactose-fermenting look-alikes like Citrobacter freundii

• Proteus species that don't swarm

• Pseudomonas species that test oxidase negative
  
  

A fast, practical reminder that no single biochemical test should stand alone.

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• Questions or feedback? Email me at letstalkmicro@outlook.com

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Welcome to the very first Micro Minutes, a new quick-hit series from Let's Talk Micro designed for students, techs, and anyone who wants fast, practical microbiology insights in under 10 minutes.

In this episode, we tackle a common misconception that microbiologists can make at the bench when interpreting a single plate: MacConkey agar.

Many assume:

"If it doesn't grow on MacConkey, it's not a gram-negative rod."
"If it does grow on MacConkey, it must be a gram-negative rod."

Both are wrong — and today we break down why.

Stay connected with Let's Talk Micro:

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• Questions or feedback? Email me at letstalkmicro@outlook.com

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Brucella is a familiar name in the lab—but recent updates to the sentinel guidelines are reshaping how we approach this pathogen. In this episode, I'm joined by Dr. Laura Filkins and Dr. Paige Larkin to discuss the latest changes: updates in nomenclature, the removal of certain Brucella species from the select agent list, and the renewed emphasis on safety in the lab. We dive into taxonomy controversies, clinical features of brucellosis, and practical steps laboratorians can take to protect themselves while delivering accurate results. Whether you're a student, laboratorian, or clinician, this conversation highlights what you need to know about the evolving landscape of Brucella diagnostics and safety.

Resources from this episode:

• CDC's Brucellosis Information Page
  
  

• ASM Updates on Brucella
  
  

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• Website: letstalkmicro.com
  
  

• Questions or feedback? Email me at letstalkmicro@outlook.com
  
  

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In this episode, Dr. Pei Zhou from the Duke University School of Medicine joins the podcast to talk about LPC-233. This is a compound that shows bactericidal activity against some gram-negative rods. LPC-233 is an LpxC inhibitor. What is LpxC? How does LPC-233 works? What are some challenges of developing LpxC inhibitors? Tune in to find out.

Link to article: https://doi.org/10.1126/scitranslmed.adf5668

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Originally released in 2021, in this episode I go over anaerobes. What are some examples of anaerobes? Why can't they grow aerobically ? What is a facultative anaerobe? Tune in to learn more.

In this episode I continue talking about MALDI-TOF MS. This time I go over the advantages and disadvantages. How has MALDI-TOF helped Clinical Microbiology? How does it compare to traditional identification methods and the workflow? What about disadvantages? Tune in to find out.

This episode features a collaboration with Jame and Callum from the ID:IOTS podcast. They are two infectious disease physicians that just like Let's Talk Micro, their goal is to share information and help educate the audience. We join forces to talk about the laboratory. We start by going over what a Medical laboratory Scientist does ( I talk about what I do). We also go over questions like what do we expect to see in a laboratory requisition. Do we add media based on what the source or the requisition indicates? How can we improve communication between the laboratory and providers? Tune in to find out.

ID:IOTS podcast page: https://idiotspodcasting.buzzsprout.com

Please download and review episodes

Luego de un episodio introductorio , Luis y Maridaliz regresan nuevamente con un episodio para la serie en español . En este episodio discuten medios de cultivo. Qué son los medios de cultivo? Cómo estos medios ayudan a los microorganismos? Cuáles son selectivos? Cuáles son diferenciales? Sintonizen para aprender sobre estos medios, las condiciones que los microorganismos necesitan para crecer, y más.

This episode features a conversation with Dr. Caterina Miraglia, a professor from the University of Massachusetts Dartmouth. Dr. Miraglia published a review article about Marburg viruses in 2019, in order to educate fellow Medical Laboratory Scientists about these type of viruses. What is Marburg virus? What is Marburg virus disease? Is there any treatment or prevention? Tune in to find out.

Let's Talk Micro fue creado con el objetivo de compartir información y aprender, enseñando todo de la manera mas simple posible. Siendo fiel a esa misión , hoy comienza una serie dentro del podcast para la población que habla español . Luis Plaza, el creador y animador del podcast, se une a la Dra. Maridaliz Rodriguez para hablar de microbiología . En este episodio Luis y Maridaliz hablan de su profesión y del objetivo de esta serie.

Antimicrobial resistance (AMR) continues to grow as a global health threat, making infections harder to treat and leaving fewer options for patients. The need for new antibiotics is an urgent matter — but traditional discovery methods are slow and limited.

In this episode of Let's Talk Micro, Luis is joined by Dr. James Collins, professor at MIT and researcher at the Broad Institute, who is leading efforts to apply AI to antibiotic discovery. We discuss how his team used deep learning to uncover promising new compounds, including NG1 and DN1, which showed activity against drug-resistant Neisseria gonorrhoeae and Staphylococcus aureus. We also talk about the challenges of bringing discoveries from computer models to the clinic, and what this approach could mean for the future of the fight against superbugs.

Link to study: https://www.cell.com/cell/abstract/S0092-8674%2825%2900855-4

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This is the second part of the interview with Dr. Joel Mortensen and his experience at a hospital in Guinea. In this episode he continues talking about training laboratory personnel and teaching essential things like quality control. He also talks about issues like supplies and electricity and future plans. Are you trained in hematology and chemistry and speak French? Are you interested in helping out with training? You can email the podcast and your information will be forwarded or you can reach out to Hope Ignited. Let's Talk Micro email: letstalkmicro@outlook.comLink to Hope Ignited: https://www.hopeignited.org

This episode features an interview with Dr. Joel Mortensen. He is the Director of the Diagnostic Infectious Diseases Testing Laboratory at Cincinnati Children's Hospital. Dr. Mortensen volunteered to train laboratory personnel to perform microbiology testing at a pediatric hospital that was being built in Guinea, West Africa. Tune in to learn about this hospital, hear about his experience, the challenges he encountered, and more. This is part one of a two part series. Link to Hope Ignited: https://www.hopeignited.org

We have heard or recent cases of malaria in the United States. What better than to do an episode about it? Dr. Kenneth Gavina, from the Indiana University School of Medicine, joins the podcast to talk about it. Tune in to learn about its life cycle, transmission, prevention, its relationship to pregnancy, and more.Link to CDC's life cycle: https://www.cdc.gov/malaria/about/biology/index.htmlLink to Future of Malaria Research Symposium: https://publichealth.jhu.edu/malaria-research-institute/2023-future-of-malaria-research-symposium

Have you worked in a veterinary laboratory? What kind of training do you need? Are you able to work in one as an MLS? Have you thought about what a culture from a veterinary specimen might look like? How do you work those up? What about antimicrobials and guidelines? Kelli Maddock and Sarah Gefroh from the North Dakota State University Veterinary Diagnostic Laboratory join the podcast to answer these questions. They are Medical Laboratory Scientists with experience in human medicine that switched to veterinary medicine.

This episode is part two of the great emerging viruses talk with Dr. Ryan Relich and Benjamin Pinsky. They talk over diagnostic tests for viruses, challenges with test development, and they share their thoughts on viruses and outbreaks.

Link to the National Academies Arbovirus workshop: https://www.nationalacademies.org/our-work/mitigating-arboviral-threats-and-strengthening-public-health-preparedness-a-workshop

Link to the Pan American Society for Clinical Virology: https://www.pascv.org/default.aspx

With all the information about viruses that we see coming out, what better than an episode about emerging viruses. Dr. Benjamin Pinsky and Dr. Ryan Relich, two experienced virologists, join the podcast to talk about viruses. Tune in to learn about the different type of viruses, mode of entry, diseases, and more. This is part one of a two-part series. Link to the Clinical Virology Symposium: https://asm.org/Events/Clinical-Virology-Symposium/Home

In this episode Dr. Ryan Relich and Dr. Kenneth Gavina join the podcast to talk about an article where Francisella tularensis spp. novicida was recovered from a blood culture. Are you familiar with this organism? What are the subspecies of Francisella tularensis? What are the biochemicals tested on it and their results? While most of us might never see this organism in our careers, being aware and prepared to recognize it is essential. We have to be prepared and have a plan in place. Tune in to learn more about this organism. Link to article: https://journals.asm.org/doi/pdf/10.1128/jcm.00787-22

Season 3 kicks off with the second episode of the AMR sub-series—this time focusing on beta-lactam antibiotics. Luis and Dr. Andrea Prinzi are joined by Dr. Brian Raux, a pharmacist, to discuss beta-lactam drug classes, mechanism of action, intrinsic resistance, and CLSI guidance. A must-listen for anyone interested in antimicrobial resistance.

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Last week, we discussed early growth isolates for antimicrobial susceptibility testing (AST) and the importance of obtaining results sooner. This week, we revisit an earlier study that looked at early growth but using disk diffusion.

In this episode, I was joined by Dr. Carey-Ann Burnham, Dr. Daniel Webber, and Meghan Wallace to discuss their work. Tune in to hear how this approach could help deliver AST results faster, the challenges of adopting early growth methods, and what it means for clinical microbiology labs.

This episode was originally released in 2022 (Episode 39: "Talking disk diffusion performed on early growth").

Link to disk diffusion study: https://doi.org/10.1128/jcm.03007-20

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This episode is the first episode in what is going to be an Antimicrobial Resistance (AMR) sub-series. Dr. Andrea Prinzi joins me in this series. Together we breakdown this complex topic. In this episode we start with an overview of the state of AMR, Dr. Prinzi provides some history, and we explain what the series is going to be about. Learning this information can be challenging for professionals, but we are going to break it down as simple as we can. Knowing and understanding this information is a crucial part of our job as Medical Laboratory Scientists. We will go over classes of antibiotics, mechanisms of action, resistance mechanisms and more. Hope you tune in.

This episode was recorded while at the American Society for Microbiology's (ASM) Microbe 2023 at Houston. I go over my favorite posters, sessions and more. It was great connecting with fellow microbiologists. Great posters such as Shewanella and oysters, frogs and potential compounds, and more. Of course, as a podcaster I had to sit through an episode of Editors in conversation.

Some of you may be familiar with the popular Parasite Wonders blog. In this episode of Let's Talk Micro, Luis is joined by its creator, Dr. Bobbi Pritt, Director of the Parasitology Laboratory at Mayo Clinic.

They dive into key questions: What specimens are typically tested for parasites? What testing methods are used? Is there a role for digital parasitology?

Dr. Pritt shares her insights and expertise on all things parasitic. Curious to learn more? Tune in to this fascinating conversation!

 Sign up for the workshop....

Link to blog: https://parasitewonders.blogspot.com

Link to website: http://parasitewonders.com/

Link to workshop: https://news.mayocliniclabs.com/2023-parasitology-workshop/

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This episode is the conclusion of Dr. Wojewoda's story about her hospital's network going down. What was the overall effect of the network going down?What happened when the system came back up? Any lessons learned and advice? Tune in to find out.

Questions? Feedback? Send those to letstalkmicro@outlook.com

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Those of us that work in the laboratory have experienced a downtime at some point or another. They tend to be about 4 hours long. What if the downtime went on for days? How would you perform tasks such as add-on testing or referencing cultures? What if the whole system was down? Dr. Christina Wojewoda from the University of Vermont Medical Center joins the podcast to tell her "horror" story of when the network at her hospital went down. This is part 1 of 2 episodes.

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This episodes features a conversation with Dr. Romney Humphries and Dr. April Abbott. They join the podcast to talk about a study where Piperacillin-Tazobactam (TZP) was evaluated. Testing results from automated systems were compared to a reference method. How did the systems perform? What are the challenges? What are CLSI breakpoints and FDA breakpoints? What is the MERINO trial? Tune in to find out. Link to article:

https://journals.asm.org/doi/10.1128/jcm.01617-22

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In this episode of Let's Talk Micro, Dr. Jennifer Dien Bard and Dr. Lucas Osborn discuss their recent study on early growth antimicrobial susceptibility testing (AST). Traditional AST often requires 18–24 hours of incubation of isolates, delaying targeted treatment. Their research shows that using 6-hour early growth isolates with both automated and manual methods delivers accurate results up to 18 hours faster—without added costs or specialized equipment.

The conversation explores the study design, agreement rates with standard methods, and what faster AST means for patient care and antimicrobial stewardship.

 Link to study: https://doi.org/10.1128/jcm.00236-25

Link to disk diffusion study: https://doi.org/10.1128/jcm.03007-20

Check out the website: https://www.letstalkmicro.com/

Questions? Feedback? Send those to letstalkmicro@outlook.com

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• Health Alert Network (HAN) - 00486 | Increase in Extensively Drug-Resistant Shigellosis in the United States (cdc.gov)
• Webinar Tuesday, February 28, 2023 - Epidemiology, Testing, and Management of Extensively Drug-Resistant Shigellosis (cdc.gov)
• Information for Healthcare Professionals | Shigella – Shigellosis | CDC
• Health Promotion Materials | Shigella – Shigellosis | CDC

The Clinical Microbiology Procedures Handbook (CMPH)—a cornerstone resource for clinical labs—has just released its 5th edition. In this episode of Let's Talk Micro, I'm joined by the editors-in-chief, Dr. Amy Leber and Dr. Carey-Ann Burnham, to discuss what's new, what's essential, and why the CMPH continues to be a trusted reference in clinical microbiology. What role does it play in the lab? How has it evolved? Tune in to find out.

📘 Link to ClinMicroNow below

Link to ClinMicroNow:

 https://onlinelibrary.wiley.com/doi/book/10.1002/9781683670438

Questions? Feedback? Send those to letstalkmicro@outlook.com

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In this episode, Dr. Andrea Prinzi and Dr. Rodney Rohde join the podcast to discuss the article 'The Role of Bacterial Biofilms in Antimicrobial Resistance.' What exactly are biofilms? Which organisms are commonly associated with them? And how do they contribute to antimicrobial resistance (AMR)? Tune in for an insightful conversation on this important topic.

Link to article:  https://asm.org/Articles/2023/March/The-Role-of-Bacterial-Biofilms-in-Antimicrobial-Re

Link to quorum sensing article: https://asm.org/Articles/2020/June/How-Quorum-Sensing-Works

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The Centers for Disease Control and Prevention (CDC) released a press release on March 20, 2023 about Candida auris. There is an increase in cases, so it is a good time to talk about it. Dr. Shawn Lockhart, from the Mycotic Diseases Branch of the CDC joins the podcast to talk about C. auris. What is C. auris? How is it transmitted? What kind of antimicrobial resistance does it exhibit? Tune in to this episode to find out.

Link to EPA's P list: https://www.epa.gov/pesticide-registration/list-p-antimicrobial-products-registered-epa-claims-against-candida-auris

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This is part 2 of a two part series. Dr. Rachael Liesman goes over scenarios where genotypic and phenotypic testing do not match and what to do. What do you do when your molecular instrument detects mec-A and Staphylococcus aureus but your susceptibility report shows oxacillin susceptible? What about VanA and Enterococcus? As Medical Laboratory Scientists in Microbiology, we encounter this type of situations. Tune in to find out how to troubleshoot these discrepancies.

Link to Dr. Patricia Simner's article: https://journals.asm.org/doi/full/10.1128/JCM.00138-20

Those of you that work in Clinical Microbiology have seen at one point that a molecular test detects a gene for antimicrobial resistance and the susceptibility profile does not match. What do we do then? In this episode Dr. Rachael Liesman joins to the podcast to talk about this. What is genotypic testing? What is phenotypic testing? How do we perform these in the lab? How do we troubleshoot these discrepancies? Tune in to this episode which is the first of a two part series to find out.

Link to Dr. Liesman's presentation: https://drive.google.com/file/d/1dphVN79alVfQERTL_rTihUA-zlqBOJfY/view?usp=share_link

This episode features an interview with the first graduate from the Doctorate in Clinical Laboratory Sciences (DCLS) program from Rutgers University: Dr. Brandy Gunsolus. What is the DCLS program? What are the requirements for this degree? What does a Doctor in Clinical laboratory Sciences does? Can they be directors? Tune in to find out about this career path for laboratorians and its contribution in Laboratory Medicine.

Link to DCLS programs:

https://shp.utmb.edu/clls/dcls/home

https://shp.rutgers.edu/clinical-lab-and-imaging-sciences/doctorate-of-clinical-lab-science/

https://www.kumc.edu/school-of-health-professions/academics/departments/clinical-laboratory-sciences/academics/doctorate-in-cls-program.html

This week, Luis is joined by Dr. Ashlan Kunz-Coyne to discuss some of the newest antibiotics for urinary tract infections (UTIs). They explore why extended-spectrum beta-lactamases (ESBLs) are a growing concern, the impact of E. coli sequence type 131, and where new agents like cefepime–enmetazobactam, sulopenem, and tebipenem fit into treatment. The conversation also highlights clinical data, stewardship considerations, and how the infectious diseases and microbiology communities can work together to preserve these options. Plus, Dr. Kunz-Coyne reveals her favorite bug—Stenotrophomonas maltophilia—and why it's unlike any other.

 Link to sulopenem episode: https://directory.libsyn.com/episode/index/id/35868135

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In this episode we talk about a relationship between bacteria and the Hawaiian bobtail squid. Dr. Sarah McAnulty and Dr. Spencer Nyholm join the podcast to talk about a study about this squid and bacteria. What is symbiosis? What is the accessory nidamental gland? How does a bacterial community contribute to the development of this gland? What bacteria make up this community? Tune in to find out.

Link to article: https://journals.asm.org/doi/10.1128/mbio.02131-22

Link to Skype a Scientist: https://www.skypeascientist.com/meet-us.html

The show "The last of us" has brought the topic of fungal infections to the spotlight. What is Cordyceps? Why is the the term zombie used? What is the relationship between fungi and body temperature? Dr. Andrej Spec, an Infectious Diseases physician, joins the podcast to talk about Cordyceps and fungi.

This week's episode is about one species of Bacillus that causes disease and has the potential of being used as a bioterror agent : Bacillus anthracis. What is anthrax? How is this organism identified in the lab? What about Bacillus cereus biovar anthracis? Tune in to this episode to find out the answers.