No Time To Read – Détails, épisodes et analyse

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No Time To Read

Arif Ashraf

Sciences

Fréquence : 1 épisode/46j. Total Éps: 40

Arif, plant biologist and host of the podcast, will talk to lead author of a recently published plant biology paper. The guest will simply explain the story of the publication, answer questions from the host, and share personal experience and details related to the article. As an audience, you will tune in to the episode with an expectation that you will know the story of the paper without reading it. Besides, you can keep listening the podcast during your experiment, walking outside, in your car and wherever possible.

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https://twitter.com/aribidopsis
21 partages
https://twitter.com/Grey_Monroe/status/1481298489191837701
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https://twitter.com/LauraBacete/status/1478342822780674053
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S3E7 | Hailong Yang | Translational landscape in a maize microRNA biogenesis mutant

Saison 3 · Épisode 7

samedi 19 octobre 2024 • Durée 27:48

No Time To Read podcast

S3E7

Translational landscape in a maize microRNA biogenesis mutant

Guest: Hailong Yang, Bartlett lab, University of Massachusetts Amherst

Previously at Beth Thompson’s lab, East Carolina University

Twitter/X: @hailongcorn

Host: Arif Ashraf, Assistant Professor, Department of Biology, Howard University

Twitter/X: @aribidopsis

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S3E6 | Natalie Hoffmann | Cell wall integrity and endomembrane trafficking

Saison 3 · Épisode 6

samedi 28 septembre 2024 • Durée 18:08

No Time To Read podcast

S3E6

Cell wall integrity and endomembrane trafficking

Guest: Natalie Hoffmann, McFarlane lab, University of Toronto

Host: Arif Ashraf, Assistant Professor, Department of Biology, Howard University

Twitter/X: @aribidopsis

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S2E7 | Katie Murphy | Specialized diterpenoid metabolism in maize

Saison 2 · Épisode 7

dimanche 21 mai 2023 • Durée 17:43

Article: A dolabralexin-deficient mutant provides insight into specialized diterpenoid metabolism in maize

Journal: Plant Physiology

Year: 2023

Guest: Katie Murphy

Host: Arif Ashraf

Abstract

Two major groups of specialized metabolites in maize (Zea mays), termed kauralexins and dolabralexins, serve as known or predicted diterpenoid defenses against pathogens, herbivores, and other environmental stressors. To consider the physiological roles of the recently discovered dolabralexin pathway, we examined dolabralexin structural diversity, tissue-specificity, and stress-elicited production in a defined biosynthetic pathway mutant. Metabolomics analyses support a larger number of dolabralexin pathway products than previously known. We identified dolabradienol as a previously undetected pathway metabolite and characterized its enzymatic production. Transcript and metabolite profiling showed that dolabralexin biosynthesis and accumulation predominantly occur in primary roots and show quantitative variation across genetically diverse inbred lines. Generation and analysis of CRISPR-Cas9-derived loss-of-function Kaurene Synthase-Like 4 (Zmksl4) mutants demonstrated dolabralexin production deficiency, thus supporting ZmKSL4 as the diterpene synthase responsible for the conversion of geranylgeranyl pyrophosphate precursors into dolabradiene and downstream pathway products. Zmksl4 mutants further display altered root-to-shoot ratios and root architecture in response to water deficit. Collectively, these results demonstrate dolabralexin biosynthesis via ZmKSL4 as a committed pathway node biochemically separating kauralexin and dolabralexin metabolism, and suggest an interactive role of maize dolabralexins in plant vigor during abiotic stress.

S2E6 | Norman Best | Maize Tassel Development

Saison 2 · Épisode 6

samedi 11 mars 2023 • Durée 10:20

Article: Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins
Journal: The Plant Journal
Year: 2022
Guest: Norman Best
Host: Arif Ashraf

Abstract

The plant hormone gibberellin (GA) impacts plant growth and development differently depending on the developmental context. In the maize (Zea mays) tassel, application of GA alters floral development, resulting in the persistence of pistils. GA signaling is achieved by the GA-dependent turnover of DELLA domain transcription factors, encoded by dwarf8 (d8) and dwarf9 (d9) in maize. The D8-Mpl and D9-1 alleles disrupt GA signaling, resulting in short plants and normal tassel floret development in the presence of excess GA. However, D9-1 mutants are unable to block GA-induced pistil development. Gene expression in developing tassels of D8-Mpl and D9-1 mutants and their wild-type siblings was determined upon excess GA3 and mock treatments. Using GA-sensitive transcripts as reporters of GA signaling, we identified a weak loss of repression under mock conditions in both mutants, with the effect in D9-1 being greater. D9-1 was also less able to repress GA signaling in the presence of excess GA3. We treated a diverse set of maize inbred lines with excess GA3 and measured the phenotypic consequences on multiple aspects of development (e.g., height and pistil persistence in tassel florets). Genotype affected all GA-regulated phenotypes but there was no correlation between any of the GA-affected phenotypes, indicating that the complexity of the relationship between GA and development extends beyond the two-gene epistasis previously demonstrated for GA and brassinosteroid biosynthetic mutants.

Cover art design and audio editing: Ragib Anjum

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S2E5 | Rachel Shahan | Arabidopsis Root Cell Atlas

Saison 2 · Épisode 5

samedi 11 février 2023 • Durée 22:45

Article: A single-cell Arabidopsis root atlas reveals developmental trajectories in wild-type and cell identity mutants
Journal: Developmental Cell
Year: 2022
Guest: Rachel Shahan
Host: Arif Ashraf

Abstract

In all multicellular organisms, transcriptional networks orchestrate organ development. The Arabidopsis root, with its simple structure and indeterminate growth, is an ideal model for investigating the spatiotemporal transcriptional signatures underlying developmental trajectories. To map gene expression dynamics across root cell types and developmental time, we built a comprehensive, organ-scale atlas at single-cell resolution. In addition to estimating developmental progressions in pseudotime, we employed the mathematical concept of optimal transport to infer developmental trajectories and identify their underlying regulators. To demonstrate the utility of the atlas to interpret new datasets, we profiled mutants for two key transcriptional regulators at single-cell resolution, shortroot and scarecrow. We report transcriptomic and in vivo evidence for tissue trans-differentiation underlying a mixed cell identity phenotype in scarecrow. Our results support the atlas as a rich community resource for unraveling the transcriptional programs that specify and maintain cell identity to regulate spatiotemporal organ development.

Cover art design and audio editing: Ragib Anjum

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S2E4 | Guangchao Sun | Genome sequence of Paspalum vaginatum

Saison 2 · Épisode 4

samedi 28 janvier 2023 • Durée 26:41

Article: Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass
Journal: Nature Communications
Year: 2022
Guest: Guangchao Sun
Host: Arif Ashraf

Abstract

A number of crop wild relatives can tolerate extreme stress to a degree outside the range observed in their domesticated relatives. However, it is unclear whether or how the molecular mechanisms employed by these species can be translated to domesticated crops. Paspalum (Paspalum vaginatum) is a self-incompatible and multiply stress-tolerant wild relative of maize and sorghum. Here, we describe the sequencing and pseudomolecule level assembly of a vegetatively propagated accession of P. vaginatum. Phylogenetic analysis based on 6,151 single-copy syntenic orthologues conserved in 6 related grass species places paspalum as an outgroup of the maize-sorghum clade. In parallel metabolic experiments, paspalum, but neither maize nor sorghum, exhibits a significant increase in trehalose when grown under nutrient-deficit conditions. Inducing trehalose accumulation in maize, imitating the metabolic phenotype of paspalum, results in autophagy dependent increases in biomass accumulation.

Cover art design and audio editing: Ragib Anjum

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S2E3 | Marta Laskowski | Root Meristem & Lateral Root

Saison 2 · Épisode 3

samedi 14 janvier 2023 • Durée 17:29

Article: The miR156 juvenility factor and PLETHORA 2 form a regulatory network and influence timing of meristem growth and lateral root emergence
Journal: Development
Year: 2022
Guest: Marta Laskowski
Host: Arif Ashraf

Abstract

Plants develop throughout their lives: seeds become seedlings that mature and form fruits and seeds. Although the underlying mechanisms that drive these developmental phase transitions have been well elucidated for shoots, the extent to which they affect the root is less clear. However, root anatomy does change as some plants mature; meristems enlarge and radial thickening occurs. Here, in Arabidopsis thaliana, we show that overexpressing miR156A, a gene that promotes the juvenile phase, increased the density of the root system, even in grafted plants in which only the rootstock had the overexpression genotype. In the root, overexpression of miR156A resulted in lower levels of PLETHORA 2, a protein that affects formation of the meristem and elongation zone. Crossing in an extra copy of PLETHORA 2 partially rescued the effects of miR156A overexpression on traits affecting root architecture, including meristem length and the rate of lateral root emergence. Consistent with this, PLETHORA 2 also inhibited the root-tip expression of another miR156 gene, miR156C. We conclude that the system driving phase change in the shoot affects developmental progression in the root, and that PLETHORA 2 participates in this network.

Cover art design and audio editing: Ragib Anjum

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S2E2 | Jenn Brophy | Synthetic Biology in Plant

Saison 2 · Épisode 2

samedi 24 décembre 2022 • Durée 20:01

Article: Synthetic genetic circuits as a means of reprogramming plant roots
Journal: Science
Year: 2022
Guest: Jenn Brophy
Host: Arif Ashraf

Abstract

The shape of a plant’s root system influences its ability to reach essential nutrients in the soil and to acquire water during drought. Progress in engineering plant roots to optimize water and nutrient acquisition has been limited by our capacity to design and build genetic programs that alter root growth in a predictable manner. We developed a collection of synthetic transcriptional regulators for plants that can be compiled to create genetic circuits. These circuits control gene expression by performing Boolean logic operations and can be used to predictably alter root structure. This work demonstrates the potential of synthetic genetic circuits to control gene expression across tissues and reprogram plant growth.

Cover art design and audio editing: Ragib Anjum

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S2E1 | Ahmet Bakirbas | Can of Spinach

Saison 2 · Épisode 1

samedi 26 novembre 2022 • Durée 19:06

Article: CAN OF SPINACH, a novel long non-coding RNA, affects iron deficiency responses in Arabidopsis thaliana
Journal: Frontiers in Plant Science
Year: 2022
Guest: Ahmet Bakirbas
Host: Arif Ashraf

Abstract

Long non-coding RNAs (lncRNAs) are RNA molecules with functions independent of any protein-coding potential. A whole transcriptome (RNA-seq) study of Arabidopsis shoots under iron sufficient and deficient conditions was carried out to determine the genes that are iron-regulated in the shoots. We identified two previously unannotated transcripts on chromosome 1 that are significantly iron-regulated. We have called this iron-regulated lncRNA, CAN OF SPINACH (COS). cos mutants have altered iron levels in leaves and seeds. Despite the low iron levels in the leaves, cos mutants have higher chlorophyll levels than WT plants. Moreover, cos mutants have abnormal development during iron deficiency. Roots of cos mutants are longer than those of WT plants, when grown on iron deficient medium. In addition, cos mutant plants accumulate singlet oxygen during iron deficiency. The mechanism through which COS affects iron deficiency responses is unclear, but small regions of sequence similarity to several genes involved in iron deficiency responses occur in COS, and small RNAs from these regions have been detected. We hypothesize that COS is required for normal adaptation to iron deficiency conditions.

Cover art design and audio editing: Ragib Anjum

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S1E12 | Adrien Burlacot | Alternative Photosynthesis

Saison 1 · Épisode 12

samedi 2 juillet 2022 • Durée 31:55

Article: Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism
Journal: Nature
Year: 2022
Guest: Adrien Burlacot
Host: Arif Ashraf

Abstract

Global photosynthesis consumes ten times more CO2 than net anthropogenic emissions, and microalgae account for nearly half of this consumption. The high efficiency of algal photosynthesis relies on a mechanism concentrating CO2 (CCM) at the catalytic site of the carboxylating enzyme RuBisCO, which enhances CO2 fixation. Although many cellular components involved in the transport and sequestration of inorganic carbon have been identified how microalgae supply energy to concentrate CO2 against a thermodynamic gradient remains unknown. Here we show that in the green alga Chlamydomonas reinhardtii, the combined action of cyclic electron flow and O2 photoreduction—which depend on PGRL1 and flavodiiron proteins, respectively—generate a low luminal pH that is essential for CCM function. We suggest that luminal protons are used downstream of thylakoid bestrophin-like transporters, probably for the conversion of bicarbonate to CO2. We further establish that an electron flow from chloroplast to mitochondria contributes to energizing non-thylakoid inorganic carbon transporters, probably by supplying ATP. We propose an integrated view of the network supplying energy to the CCM, and describe how algal cells distribute energy from photosynthesis to power different CCM processes. These results suggest a route for the transfer of a functional algal CCM to plants to improve crop productivity.

Art credit: Solène Moulin
Cover art design and audio editing: Ragib Anjum

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No Time To Read – Détails, épisodes et analyse

Détails du podcast

Classements récents

Apple Podcasts

Spotify

Liens partagés entre épisodes et podcasts

Twitter22

Other21

Qualité et score du flux RSS

Historique des publications

Derniers épisodes publiés

Podcasts et contenus similaires

Podcasts Similaires Basées sur le Contenu

Apple Podcasts

Spotify

Podcasts Similaires Basées sur le Contenu

Twitter22

Other21

Nature7