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TitreDateDurée
8:3. DNA Libraries and Generating cDNA15 Aug 202000:04:58
Prot —> mRNA —> cDNA —> dsDNA. (Step 2 via reverse transcriptase and step 3 via DNA Polymerase). Now the dsDNA is injected into a virus which infects bacteria to make lots of the DNA.
8:2. Polymerase Chain Reaction15 Aug 202000:06:57
Denaturation (Heat to high temp), Primer Annealing (Cool slightly), Primer Extension via polymerase (Heat but not as much as step 1).
8:1. Gel Electrophoresis15 Aug 202000:06:31
GE find us the length of a DNA Strand (how many base pairs long).
7:5. Applying the HP Equation10 Aug 202000:01:41
Not really sure why I did a module on this - basically you know they’re homo recessive because they have blue eyes (the recessive trait), and you know to use q BECAUSE we’re dealing with the recessive trait.
7:4. The Hardy-Weinberg Equation10 Aug 202000:02:40
HP Equation gives P(allele frequencies). P^2 is probability of homo dominant, Q^2 is probability of homo recessive, 2pq is probability of heterozygous allele combo. P = frequency of dom allele, Q = frequency of recessive allele.
7:3. Worked Example: Punnett Squares10 Aug 202000:06:05
Discusses Dihybrid Crosses and the Law of Independent Assortment.
7:2. Co-dominance and Incomplete Dominance10 Aug 202000:00:59
Don’t let the title fool you. This is short. Discusses complete, incomplete, and co-dominance.
7:1. An Introduction to Mendelian Genetics10 Aug 202000:06:47
Discuss genes, alleles, geno/phenotypes, and punnet squares. The Khan Academy video discusses the history (Gregor Mendel) but let’s be real, who cares.
6:5. The Effects of Mutations10 Aug 202000:02:14
Mutations can be good, bad, or both.
6:4. Mutagens and Carcinogens10 Aug 202000:08:19
Define mutagens and then discuss endogenous and exogenous mutagens. (Endo: ROS); (Exo: intercalators, base analogues, carcinogens)
6:3. The Causes of Genetic Mutations04 Aug 202000:10:46
Small scale mutations (point: TTM; frame-shift: ID) and Large-scale mutations (translocation, inversion).
6:2. The Different Types of Mutations04 Aug 202000:08:16
Point, Frame Shift, and Nonsense vs. Missense. Further divided Missense to Silent, Conservative, and Non-Conservative.
6:1. Introduction to Genetic Mutations03 Aug 202000:04:58
Clarifies that mutations arise at the DNA level, and from where mutations originate.
5:7. Tumor Suppressors03 Aug 202000:09:25
Discusses categories of tumor suppressors and then 2 examples: pRb and p53, both proteins.
5:6. Oncogenes03 Aug 202000:21:40
Discusses the 3 mechanisms through which oncogenes are formed from proto-oncogenes, and types of oncogenes with examples.
5:5. Non-Coding RNA26 Jul 202000:13:24
I discuss ncRNAs (RNA that’s ready to perform tasks w/o becoming protein) and then move onto rRNA, tRNA, miRNA, snRNA, and snoRNA. Have fun with all these acronyms lol
5:4. Post-Transcriptional Regulation20 Jul 202000:08:58
Not really an important module but skip to 6:18 for new stuff (RNA Editing via the ADAR and CDAR Enzymes)
5:3. Regulation of Transcription19 Jul 202000:17:27
Discusses GTFs, then discusses activators/repressors and enhancers/silencers. Finally distinguishes between regulation of transcriptional euk and prok organisms.
5:2. DNA and Chromatin Regulation18 Jul 202000:17:38
Discusses HAT and HDAC enzymes to uncoil/coil DNA (uncoil = activate for transcription) then transitioned into DNA methylation as it relates to CpG Islands and MBB’s.
5:1. The Lac Operon - Part 230 Jun 202000:13:24
More terminology of the Lac Operon mechanism, refined ideas from the first part.
4:16. DNA Structure and Function (Random Info)30 Jun 202000:08:15
End of chapter random info (as I’ve done before). Although pay attention to end where I discuss donor:acceptor ratios of nucleobases.
4:15. The Jacob Monod Lac Operon29 Jun 202000:14:40
Discusses why gene expression regulation occurs @ transcription level. Discusses the Jacob Monod Lac Operon’s structure (ZYA) and function (lactose metabolism) and how the repressor (@operator site) controls this process.
4:14. Protein Modification27 Jun 202000:30:51
LISTEN @ 2x speed (on Apple podcasts). Discusses co- and post-translational modifications (GLUMPP) which occur for those proteins that aren’t fully ready to work even after translation finishes.
4:13. Semi-Conservative Replication26 Jun 202000:02:42
Discusses the conservative (Incorrect), dispersive(Incorrect), and Semi-Conservative (Correct) theories of replication.
4:12. DNA Repair 2 (response to Dimers) + Summary of BOTH DNA Repairs26 Jun 202000:18:28
Discuss endo- and exogenous factors that damage DNA, the process of Nucleotide Excision Repair (NER), and what happens when NER fails.
4:11. DNA Repair 1 (response to Mutations)24 Jun 202000:19:21
(This is where the modules get longer/content heavy). Discusses exo- and endonucleases as well as the Mismatch Repair Mechanism as a physiological fail safe.
4:10. Differences in translation between prokaryotes and eukaryotes22 Jun 202000:09:59
Strand structure, protection factors (proks have none), and starting A2 (proks = fmet // euks = met)
4:9. Translation21 Jun 202000:07:50
Discusses the process using the APE Sites.
4:8. Speed and Precision of DNA Replication21 Jun 202000:00:44
USELESS MODULE // I didn’t think I’d actually get it to 45 seconds LOL
4:7. Transcription and mRNA Processing21 Jun 202000:15:02
I know we talked about this in 4:1 but this has more detail. Distinguishes between eukaryotic and prokaryotic transcription and reviews eukaryotic transcription in detail (with processing).
4:6. The DNA Replication Process20 Jun 202000:04:54
(Yes it has a different name). The 8-step process.
4:5. Telomeres and Single Copy DNA vs. Repetitive DNA 19 Jun 202000:11:35
Introduce telomeres as “protective caps” to ensure protection and quality replication of DNA sequences. Also discuss single copy DNA (important for genetic info) vs repetitive DNA (telomeres are an example of this).
4:4. Antiparallel Structure of DNA Strands19 Jun 202000:03:39
The strands (each of the 2 sugar phosphate backbones) run in opposite directions. That is all.
4:3. Molecular Structure of DNA19 Jun 202000:04:01
Really just a recap of the previous module + the types of bonding that forms the Double Helix
4:2. DNA (Overview)19 Jun 202000:04:47
Discuss DNA’s basic structure as well as the nitrogenous base pairs.
4:1. Eukaryotic gene transcription (DNA —> RNA)16 Jun 202000:18:28
(Content heavy!) Transcription, Structural Differences of Nucleic Acids, mRNA Processing, and RNA Interference
3:7. The Lineweaver-Burke Plot15 Jun 202000:01:31
Search up the Lineweaver-Burke Plot on your own.
3:6. Covalent Modifications to Enzynes15 Jun 202000:11:33
Covalent Modifications are changes made to a protein to accomplish a variety of tasks (activate, deactivate, etc). I discuss Small Post-translational Modifications, Zymogens, and Suicide Inhibitors.
3:5. Non-enzymatic Protein Function15 Jun 202000:11:19
MARTI (Motor, Antibodies, Receptors, Transport, and Ion Channel) Proteins. Don’t forget LITHOL for types of enzymes.
3:4. Allosteric Regulation and Feedback Loops14 Jun 202000:15:22
Discuss allostery, feedback loops, and the phosphofructokinase enzyme and it how it is involved in a feedback loop. (Pretty long module)
3:3. Cooperativity (or Cooperative Binding)11 Jun 202000:08:04
Details positive, negative, and noncooperation binding.
3:2. Steady States and the Michaelis-Mentin Equation11 Jun 202000:12:04
Discusses the steady state assumption and its equation, the MM Equation, and the catalytic efficiency equation. Also notes Kcat and Km and their interpretations.
3:1. An Introduction to Enzymes Kinetics11 Jun 202000:10:27
I discuss the 3 assumptions that allow for the (many) conclusions we will reach later in this season.
2:7. Enzymes and their Local Environment11 Jun 202000:05:05
The effects of the pH and Temperature factors on catalysis.
2:6. Cofactors, Coenzymes, and Vitamins11 Jun 202000:05:55
What they are and some examples. Discuss his minerals as well.
2:5. Six Types of Enzymes11 Jun 202000:11:52
LITHOL // Transferase, ligase, oxidoreductase, isomerase, hydroplane, lyase
2:4. Induced Fit Model of Enzyme Catalysis09 Jun 202000:07:59
Details active and allosteric site binding and distinguishes between reaction and regulation.
2:3. Enzymes and Activation Energy09 Jun 202000:04:59
Details how catalysts affect a reaction.
2:2. Intro to Enzymes and Catalysts08 Jun 202000:04:52
It’s in the title.
2:1. Enzyme Structure and Function08 Jun 202000:01:47
Discusses catalytic conditions and collision theory.
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