In this enlightening episode of Concentrating on Chromatography, host David Oliva engages in a comprehensive discussion with Lisa Jones, Director of Higher Education Relations at I.W. Tremont Co. and active NAOSMM volunteer, alongside Jim Averso, Co-President at I.W. Tremont and producer of the LabExact brand of laboratory consumables.

The conversation explores critical aspects of laboratory supply management, including best practices for procurement in academic settings, inventory challenges, and the advantages of American-made products. Lisa shares valuable insights from her 27 years of experience at the University of Alabama, highlighting how institutional policies affect purchasing decisions and the complexities of managing laboratory stockrooms. Jim discusses how I.W. Tremont develops products based on real laboratory workflows and responds to researcher feedback.

Key topics include:

• The role of NAOSMM (National Association of Scientific Materials Managers) in connecting laboratory managers with quality suppliers

• Challenges of cylinder-based nitrogen supply versus on-demand nitrogen generation

• The importance of vendor selection criteria including quality certification, inventory availability, and customization capabilities

• How academic procurement processes vary between institutions and departments

• The value of American manufacturing and family-owned businesses in the scientific supply chain

Whether you're managing university laboratory resources, working in a research facility, or supplying the scientific community, this episode offers practical perspectives on streamlining your supply chain while maintaining research quality and addressing the unique challenges of laboratory operations.

#LabSupplies #AcademicResearch #LabManagement #NAOSMM #ScientificProcurement #ChemistryLab #LabExact

In this episode of Concentrating on Chromatography, host David Oliva interviews Jim Averso, Co-President of I.W. Tremont and manufacturer of the LabExact brand, celebrating their 45th year in business. Jim shares expert insights on the critical role of filtration in chromatography sample preparation.Discover:- Why proper filtration is essential for protecting your expensive HPLC columns- How to select the right membrane type (Nylon, PTFE, PVDF, or PES) based on your sample- Common mistakes researchers make with syringe filters and how to avoid them- The innovative TruPor membrane technology that increases loading capacity while improving flow rates- Tips for preventing membrane intrusion and back pressure issues- The new HandyPrep device that simplifies pre-filtration steps- Advanced techniques like filter stacking for improved sample preparationWhether you're working with HPLC, UHPLC, GC, or other chromatography methods, this conversation provides valuable insights to optimize your workflow and improve analytical results.Jim also discusses the Laboratory Products Association's student engagement committee, which is building bridges between academia and commercial science careers.#Chromatography #LabTechniques #SamplePreparation #HPLC #Filtration #LabExact #ScienceEducation

Dive into the cutting-edge world of pharmaceutical analysis with Mark Dennis Retrato, a PhD candidate at Uppsala University. In this exclusive interview, Mark shares his groundbreaking research on developing a novel UPLC-MS method for analyzing phospholipids in total parenteral nutrition (TPN) products. Key highlights: • Innovative UPLC-MS method for phospholipid quantification • Importance of nitrogen evaporation in sample preparation • Unexpected findings in phosphatidylcholine species • Implications for quality control in pharmaceutical manufacturing • Potential impact on patient care and TPN product stability Learn how this research is pushing the boundaries of analytical chemistry and contributing to improved healthcare outcomes. Whether you're a scientist, healthcare professional, or simply curious about advancements in medical technology, this interview offers valuable insights into the future of parenteral nutrition analysis. Don't forget to like, subscribe, and hit the notification bell to stay updated on the latest developments in chromatography and mass spectrometry! #UPLCMS #ParenteralNutrition #PharmaceuticalAnalysis #Chromatography #MassSpectrometry #PhDResearch

Join Organomation's General Manager David Oliva as he interviews Bianca Costa, a PhD researcher from the University of Southern California, about her groundbreaking work on PFAS (per- and polyfluoroalkyl substances). In this in-depth discussion, Bianca shares her experiences and insights on: • The complexities of PFAS sampling and analysis in various environmental matrices • Challenges in method development for PFAS extraction and detection • The use of advanced analytical techniques like LC-MS for PFAS research • Emerging biotechnologies for PFAS remediation and the potential for microbial transformation • Community engagement efforts to assess PFAS exposure in Southern California Discover the latest advancements in PFAS research and the interdisciplinary approach needed to tackle this widespread environmental challenge. Whether you're a researcher, environmental professional, or simply interested in cutting-edge science, this interview offers valuable insights into the world of PFAS and environmental chemistry. #PFAS #EnvironmentalChemistry #WaterQuality #AnalyticalChemistry #Chromatography #Biotechnology #USC

Join us for an interesting interview with Taylor Bias, a leading researcher in the field of mitochondrial proteins, as she discusses her groundbreaking work on mitoNEET and its implications for diseases like Type 2 Diabetes, Parkinson's Disease, and Cancer. In this engaging conversation with Organomation General Manager David Oliva, Taylor shares the challenges she faced in developing analytical methods to study mitoNEET's enzymatic activities and the pivotal role of chromatography techniques like GC-MS and HPLC in her research. Discover how chromatography is evolving in the study of complex proteins and hear about some unexpected findings that have emerged from Taylor's research. For young researchers eager to dive into interdisciplinary studies, Taylor offers valuable advice on navigating this exciting field. Additionally, she addresses common misconceptions about mitoNEET and clarifies its significant role in disease mechanisms. Don't miss this opportunity to learn from an expert about the fascinating intersection of biochemistry, disease research, and advanced analytical techniques!

In this episode of "Concentrating on Chromatography," host David Oliva explores diverse applications of chromatography and mass spectrometry with researchers from the University of Nevada, Reno. The discussion covers a range of topics, including:

• PFAS research and thermal destruction processes
• Short-chain fatty acid production in gut microbiomes using GC-MS
• Cortisol analysis in sheep wool for welfare studies using ELISA
• The versatility of university core laboratories in supporting various research projects

Listeners will gain insights into cutting-edge research methodologies, sample preparation techniques, and the critical role of chromatography in environmental science, microbiology, animal welfare, and beyond. This episode highlights the interdisciplinary nature of analytical chemistry and its impact on addressing real-world challenges.

In this insightful interview, David Oliva, General Manager of Organomation, sits down with Jonathan Navarro Ramos, a PhD student at the University at Buffalo, to discuss his cutting-edge research on environmental pollutants. Jonathan shares his journey in developing methods to detect tire-derived chemicals like 6PPD and 6PPD-quinone in stormwater runoff and highlights the importance of non-target analysis using high-resolution mass spectrometry (HRMS). Key Topics Covered: Research on 6PPD and its transformation into 6PPD-quinone Environmental and public health concerns surrounding tire-derived chemicals The use of LC-MS/MS in detecting pollutants in challenging matrices Sample preparation techniques, including solid-phase extraction and nitrogen blowdown with the Organomation N-EVAP Future research directions involving the chlorination of pollutants in wastewater treatment Learn More: Jonathan also shares surprising findings from his study and emphasizes the global ubiquity of tire-derived pollutants. Don't miss this engaging discussion! 👉 Follow us for more updates on environmental research, chromatography techniques, and advanced laboratory equipment. #EnvironmentalResearch #TireChemicals #LCMS #MassSpectrometry #SamplePreparation #Organomation #PollutantDetection #PhDResearch

Join us on the Concentrating on Chromatography podcast as David Oliva interviews Katelynn Perrault Uptmor, PhD about her recent article, "Detangling the Complex Web of GC x GC Method Development to Support New Users." In this insightful conversation, Katelynn explains the basics of comprehensive two-dimensional gas chromatography (GC x GC), its advantages over traditional one-dimensional GC, and shares practical tips for overcoming common challenges faced by new users.Discover how GC x GC vastly increases separation capacity by combining two columns with different stationary phases, allowing for more efficient analysis of complex samples. Katelynn discusses the key steps in method development, including establishing a good one-dimensional separation, using chromatogram modelers to predict peak behavior, and fine-tuning modulator parameters for optimal peak shape and resolution.Learn about choosing the right column combinations for your specific application, understanding the importance of orthogonality between columns, and leveraging resources like literature and expert advice to optimize your setup. Explore real-world applications of GC x GC in fields like forensics, food analysis, environmental science, and petroleum analysis, where this technique is revolutionizing routine analysis.Whether you're a seasoned chromatographer or just starting out, this interview offers valuable insights into the power and accessibility of GC x GC. Katelynn also shares her recent recognition as an Emerging Leader in chromatography and highlights the exciting work of her undergraduate research group. Katelynn is currently Assistant Professor of Chemistry at William and Mary.

Dive into the world of cutting-edge catalysis research with Dr. Roberto Silva Villatoro in this captivating episode of Concentrating on Chromatography. Hosted by David Oliva, General Manager of Organomation, this interview explores how high-resolution mass spectrometry (HRMS) is revolutionizing our understanding of palladium-catalyzed reactions. Key highlights: - Discover how HRMS detects elusive organometallic intermediates - Learn about overcoming air-sensitivity challenges in sample preparation - Explore the implications of off-cycle species in catalytic processes - Gain insights into the future of C-X and C-C bond-forming reactions Whether you're a chemistry enthusiast, a seasoned researcher, or an industry professional, this interview offers valuable insights into the intricate world of catalytic mechanisms. Join us as we uncover the potential for cleaner, more efficient chemical processes and the power of interdisciplinary collaboration in advancing scientific discovery. Don't forget to like, subscribe, and hit the notification bell to stay updated on the latest in chromatography, mass spectrometry, and sample preparation! #Catalysis #MassSpectrometry #ChemistryResearch #Chromatography #ScientificDiscovery

Join us for an insightful interview with Dr. Isaiah Speight, Assistant Professor at William and Mary, as he discusses his groundbreaking work in sustainable chemistry and innovative chromatography techniques. In this engaging conversation, Prof. Speight shares: - His research focus on sustainable chemistry from synthesis, financial, and educational perspectives - The importance of NOBCChE (National Organization for the Professional Advancement of Black Chemists and Chemical Engineers) - How HPLC and flash chromatography enable synthetic chemistry - The advantages of automated flash chromatography systems - Method development processes for new compounds and reactions - Exciting ongoing projects, including 3D-printed mechanochemical reaction vessels and mercury-centered metal-organic frameworks Discover how Prof. Speight's lab is pushing the boundaries of sustainable chemistry while training the next generation of undergraduate researchers. This interview offers valuable insights for chemists, students, and anyone interested in the future of sustainable scientific practices. #SustainableChemistry #Chromatography #STEM #NOBCChE #ResearchInnovation

Dive into the world of environmental toxicology with Andrea Andress Huacachino, a PhD student at the University of Pennsylvania. In this fascinating interview, Andrea discusses her groundbreaking research on PFAS (per- and polyfluoroalkyl substances) and their impact on androgen regulation. Learn about: - The novel finding of PFOA's effect on pre-receptor androgen regulation - Innovative use of radio chromatography in hormone analysis - Challenges in developing HPLC methods for androgen separation - The broader implications of androgen dysregulation on human health Discover how environmental pollutants affect hormones in all humans and why this research matters for everyone. Perfect for science enthusiasts, students, and anyone interested in the intersection of chemistry and human health. #EnvironmentalToxicology #PFAS #Androgens #ChemistryResearch #ScienceInterview

Dr. Caitlin Cain discusses her groundbreaking research on potato taste defect (PTD) in coffee using comprehensive two-dimensional gas chromatography (GC×GC). Learn how this advanced technique revealed 359 class-distinguishing analytes related to PTD, providing unprecedented insights into this complex flavor issue. Key points covered: - Advantages of GC×GC over traditional one-dimensional GC for complex samples - Optimization of headspace solid-phase microextraction for coffee volatile analysis - Surprising findings from the comprehensive volatile profile of PTD-affected coffee - Implications for coffee farmers and the industry in combating PTD Discover how this research is advancing our understanding of coffee chemistry and potentially improving coffee quality worldwide. #CoffeeScience #Chromatography #AnalyticalChemistry #FoodChemistry

Dive into the cutting-edge world of natural product discovery with Jess Deng, a fourth-year PhD candidate at Queen's University. In this engaging interview, Jess discusses her groundbreaking research using ambient ionization mass spectrometry to streamline the discovery of novel fungal metabolites. Key highlights: - Learn about the liquid microjunction surface sampling probe (LMJ SSP) and its advantages - Discover how Jess is tackling the time and resource challenges in natural product research - Explore the effects of light conditions and environmental factors on fungal metabolism - Hear about Jess's exciting work at the Smithsonian Museum of Conservation From rapid screening techniques to machine learning algorithms, Jess shares insights that could reshape the future of pharmaceutical research and biofuel production. Don't miss this fascinating look into the intersection of mass spectrometry, fungal biology, and drug discovery! #MassSpectrometry #NaturalProductDiscovery #FungalMetabolites #PhDResearch #ASMS2024

In this insightful interview, Medicinal Chemist Schyler Odum discusses his work in antibiotic drug discovery and the critical role of Ultra-High Performance Liquid Chromatography (UPLC) in his research. Schyler, a fifth-year graduate student at UTHSC, shares his expertise on: The importance of antibiotic drug discovery in academic institutions How UPLC differs from HPLC and its benefits in chemical analysis Challenges in sample preparation and potential improvements The complexities of synthesizing large molecular weight antibiotics The need for increased support and funding in antibiotic research Schyler also highlights the Gordon Research Conferences as a platform for presenting antibiotic drug discovery work. This video offers valuable insights for chemistry students, researchers, and anyone interested in the future of antibiotic development. #AntibioticResearch #MedicinalChemistry #UPLC #DrugDiscovery #GraduateResearch

Dive into the world of sample preparation with industry expert Wade Hasenour as he shares invaluable insights on rotary evaporation, centrifugal evaporation, and freeze drying. Learn about the latest advancements, challenges, and future trends in sample prep techniques for chromatography. Discover how to choose the right method for your needs, optimize your workflow, and leverage automation for better results. Whether you're a seasoned chromatographer or just starting out, this video offers essential knowledge to enhance your sample preparation skills and achieve more reliable outcomes in your research. #Chromatography #SamplePreparation #LabTechniques #ScientificResearch #massspectrometry

Eugene Oga, a PhD student in Analytical Chemistry at the University of North Dakota, joins David Oliva to discuss his innovative research on silicone sealant degradation using evolved gas analysis and thermal desorption-pyrolysis gas chromatography. With his background in materials chemistry and current focus on analytical techniques, Eugene shares insights into how these advanced methods can characterize material breakdown over time, with significant implications for the construction industry and beyond.Eugene's Background and Research FocusEugene Oga is pursuing his PhD in Analytical Chemistry at the University of North Dakota, following his Bachelor's and Master's degrees from the University of Buea in Cameroon. His academic journey began with a focus on materials chemistry before transitioning to analytical chemistry, recognizing that material properties and performance depend heavily on proper characterization.His current research employs advanced analytical techniques like evolved gas analysis and pyrolysis gas chromatography to understand how materials break down over time and identify the specific fragments produced during degradation. This work aims to provide recommendations to manufacturers about potential improvements to their processes.Understanding Structural Sealant Glazing and Its ImportanceStructural Sealant Glazing (SSG) plays a critical role in modern construction, particularly in buildings that extensively use glass and metal. Eugene explains that sealants are thick liquids used to bind different construction materials together.These sealants serve multiple important functions, including preventing air infiltration, moisture penetration, and enhancing building stability. While studies have estimated how long sealants and buildings should last, they often don't explain the actual breakdown mechanisms. Eugene's research aims to fill this gap by identifying which components are lost over time and how this degradation occurs, ultimately enabling better prediction of failure and development of solutions.Different Failure Modes and Analytical ApproachesEugene identifies several categories of sealant failure that require different analytical approaches:1. Adhesive/cohesive failures: These occur when materials don't properly bind together, often due to surface preparation issues that can be addressed through proper cleaning.2. Chemical degradation: This includes processes like UV exposure, hydrolysis, and oxidation.3. Environmental factors: Temperature fluctuations, moisture, and pollutants can contribute to degradation.4. Physical degradation: Manifesting as cracking, fatigue, and creep.While mechanical analysis can identify physical changes like cracking and strength loss, it doesn't reveal which chemical components are being lost. For all these failure modes, gas chromatography and mass spectrometry prove essential.In all of these different failure modes and causes, gas chromatography and mass spectrometry would be very important to identify the volatile degradation products and the changes that are accompanying the backbone.Innovative Methodological ApproachEugene's research employs a two-step analytical approach that modifies standard GC-MS protocols to accommodate siloxane chemistry:1. Evolved Gas Analysis (EGA-MS): This serves as a rapid screening technique to identify temperature ranges where components evolve.2. Thermal Desorption-Pyrolysis GC-MS: This follows as a verification tool, focusing on the specific temperature zones identified by EGA to perform detailed analysis of components evolving at different temperatures.This sequential approach allows for more targeted analysis and better characterization of degradation products. The method is particularly valuable because it eliminates extensive sample preparation—samples can be analyzed as received, saving significant time and reducing potential preparation errors.

In this episode of Concentrating on Chromatography, host David Oliva interviews Dr. Petr Vozka, Assistant Professor at California State University, Los Angeles, about his journey establishing the Complex Chemical Composition Analysis Lab (C³AL).Dr. Vozka shares how he built his research facility during the pandemic, focusing on creating opportunities for first-generation and underrepresented undergraduate students. Learn about the groundbreaking LECO-C³AL partnership that provides students with hands-on experience using advanced comprehensive two-dimensional gas chromatography (GC×GC) instrumentation.The conversation explores:- Building a cutting-edge analytical chemistry lab during challenging circumstances- Creating research opportunities for underrepresented undergraduate students- The innovative fingerprint aging research in collaboration with the Hertzberg-Davis Forensic Science Center- How multidimensional chromatography enables detection of thousands of compounds in complex mixtures- The workflow for analyzing fingerprint samples using GC×GC-TOFMS- The advantages of nitrogen generators in laboratory settingsDr. Vozka also discusses the upcoming 17th Multidimensional Chromatography Workshop (MDCW), a FREE conference taking place January 13-15, 2026, in Williamsburg, VA, bringing together experts in multidimensional separation techniques.Visit Dr. Vozka's lab website: https://www.calstatela.edu/research/c3alLearn more about MDCW: https://www.multidimensionalchromatography.com/#Chromatography #GCGC #ForensicScience #AnalyticalChemistry #Fingerprints #MDCW #CalStateLA

Dr. Haleigh Boswell, Research Chemist at Chevron, joins the Concentrating on Chromatography podcast to discuss her fascinating career journey and cutting-edge analytical work in the energy industry. In this in-depth interview, Dr. Boswell shares:🔬 Technical Insights:- How two-dimensional gas chromatography (GCxGC) differs from traditional GC and why it's revolutionary for complex sample analysis- Applications of GCxGC in analyzing hydrocarbon and renewable matrices- Detector selection strategies (FID, SCD, NCD, TOF-MS) for different analytical challenges- Sample preparation considerations for complex energy industry matrices🎯 Industry Applications:- Real-world problem-solving in refinery operations and catalyst development- Non-targeted analysis for trace contaminants in renewable feedstocks- Process optimization and quality control in energy production- Safety considerations and instrument maintenance in industrial settings📈 Career Journey:- Transition from forensic science undergraduate studies to analytical chemistry- Moving from academia to industry: challenges and adaptations- The importance of networking in scientific careers- Advice for graduate students considering industry positions🏢 Behind the Scenes at Chevron:- Day-to-day responsibilities as a subject matter expert (SME)- Collaborative work across multiple R&D teams- Modernizing analytical instrumentation in a legacy company- Balancing innovation with business needs and safety requirementsThis episode provides valuable insights for analytical chemists, graduate students, and anyone interested in the practical applications of advanced chromatography techniques in industrial settings[#Chromatography #AnalyticalChemistry #EnergyIndustry #GCxGC #CareerAdvice #ChemistryPodcast

Join us for an in-depth conversation with Dr. Eamonn Clark, postdoctoral researcher at the University of Hawaii,  Mānoa and the Water Resources Research Center, as he shares his journey in water research and laboratory innovation.In this episode:- Dr. Clark introduces his work on water treatment and disinfection byproducts, including research following the Red Hill fuel spill in Oahu.- A behind-the-scenes look at the development of a home-built nitrogen blowdown evaporator—why it was needed, how it was designed, and who can benefit from building their own lab equipment.- Discussion of workflows for PFAS analysis, the importance of accessible analytical tools, and the impact of DIY solutions in low-resource settings.- Insights into other homemade lab instruments, the role of 3D printing and PCB design, and the value of community science in water quality monitoring.- Reflections on the broader impact of water research, community engagement, and the importance of making science accessible to all.Key topics covered:- Water Resources Research Center and its mission- Analytical methods for PFAS and disinfection byproducts- Step-by-step overview of building a nitrogen evaporator- Who should consider DIY lab equipment- The future of homemade scientific tools and citizen science- Community outreach and the real-world impact of water researchWhether you’re a scientist, student, or simply curious about how research labs innovate on a budget, this episode offers practical advice and inspiration for building your own scientific equipment and making a difference in your community.Check out for links to Dr. Clark’s technical note and additional resources on DIY lab equipment and water research:- https://pubs.rsc.org/en/content/articlelanding/2025/ay/d4ay02118e/unauth- https://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=ay&themeid=9bc2db53-354c-4a55-84eb-93fcae875044- https://blog.organomation.com/blog/inaugural-homemade-evaporator-contest-results-the-peak-of-ingenuity

Join David Oliva on the Concentrating on Chromatography podcast as he interviews Edward Faden, Vice President and co-owner of MAC-MOD Analytical, a leading provider of chromatography solutions since 1986. In this insightful discussion, Edward shares the history and expertise of MAC-MOD, based in Chadds Ford, Pennsylvania, and dives deep into the critical challenges of analyzing PFAS (per- and polyfluoroalkyl substances), often called "forever chemicals."Key topics include:- The pervasive nature of PFAS contamination in both environmental and laboratory settings.- Surprising sources of PFAS contamination identified in standard lab setups, from gas line tubing to everyday consumables.- The essential role of the PFAS Delay Column in ensuring accurate analysis by minimizing background interference.- Best practices for labs starting PFAS analysis to reduce contamination risks from day one.- Exciting developments in rapid sample pre-screening methods to prevent cross-contamination.Edward also discusses why PFAS are so widespread—used in products like non-stick cookware and waterproof clothing since the 1940s—and the ongoing struggle to manage their environmental persistence. Whether you're a chemist, environmental scientist, or simply curious about the impact of forever chemicals, this conversation offers valuable insights into cutting-edge analytical solutions.Don't miss out on learning about MAC-MOD's innovative approaches and future plans for PFAS analysis. Subscribe for more expert interviews and chromatography content!#Chromatography #PFAS #ForeverChemicals #EnvironmentalScience #LabAnalysis

🧪 Transform Your Understanding of Modern Chromatography & AI!

Join us for an eye-opening conversation with Professor Dwight Stoll from Gustavus Adolphus College as he reveals how his team is revolutionizing liquid chromatography through high-throughput data collection and machine learning. With over 43,000 retention measurements and groundbreaking research spanning three major publications, Prof. Stoll is pioneering the future of analytical chemistry.🔬 What You'll Learn:- How short columns (5mm vs 100mm) dramatically increase measurement throughput while maintaining accuracy- The evolution from HSM1 to HSM3 models and their predictive capabilities - Why selectivity ratios are more stable than absolute retention factors- The "Manhattan Project of Chromatography" - building massive retention databases- Key challenges: column drift, mobile phase standardization, and precision trade-offs- Career advice for researchers at the intersection of chromatography and data science📊 Key Highlights:- 43,329 total retention measurements across 13 stationary phases- Revolutionary "feed injection" method for high-throughput analysis- Improved isomer selectivity predictions - crucial for pharmaceutical analysis- The holy grail: predicting retention from molecular structures🎯 Perfect For:- Analytical chemists and chromatographers- Data scientists interested in chemistry applications- Graduate students and researchers- Pharmaceutical industry professionals- Anyone curious about AI applications in science🔗 Connect with Prof. Stoll:- Gustavus Adolphus College Chemistry Department- Research focused on liquid chromatography and 2D-LC#Chromatography #MachineLearning #AnalyticalChemistry #AI #DataScience #Research #HPLC #Pharmaceutical #Chemistry #ScienceThis interview showcases cutting-edge research that's transforming how we approach method development in liquid chromatography. Don't miss this glimpse into the future of analytical chemistry!

Join us for a fascinating conversation with Dr. Chad Pozarycki, PhD in analytical chemistry and astrobiology, as we dive into the cutting-edge science behind searching for life beyond Earth. In this episode, we explore groundbreaking research on how biosignature molecules—such as amino acids and ATP—can accumulate and persist in extreme, hypersaline brine environments analogous to those found on Mars, Europa, and other planets.Dr. Pozarycki explains the unique analytical challenges of working with highly salty samples, discusses innovative techniques like capillary electrophoresis and high-resolution mass spectrometry, and offers key advice for scientists developing the next generation of space-ready instruments. If you’re curious about how planetary scientists actually analyze samples from Mars or icy moons, or are interested in the intersection of analytical chemistry, planetary exploration, and astrobiology, this episode is for you!🔬 Topics covered:- Analytical methods for detecting biomolecules in salt-rich planetary environments- Overcoming salt-interference in chromatography and mass spectrometry- Method validation in extreme conditions- Adapting scientific instruments for use on Mars rovers and Europa landers- Career and practical advice for aspiring analytical chemists and astrobiologistsDon’t miss Dr. Pozarycki’s insights on the next frontiers for miniaturized analytical instruments and what it takes to prepare for future space missions.Listen now to discover how planetary analog research on Earth paves the way for discovering life elsewhere in our solar system!#Astrobiology #AnalyticalChemistry #SpaceExploration #PlanetaryScience #Chromatography #MassSpectrometry #Biosignatures

Join Dr. Guy Caldwell, Distinguished Research Professor at the University of Alabama, for a riveting and urgent conversation that goes beyond the lab bench to the heart of America’s biomedical research crisis. In this episode, Dr. Caldwell—an internationally recognized leader in neurodegenerative disease research—shares the real-world impact of NIH funding freezes, budget cuts, and policy gridlock on labs, promising young scientists, and the future of life-changing discoveries

Hear compelling stories from both the "Worm Shack" lab and classrooms filled with top science students: top-ranked research grants on Alzheimer’s and Parkinson’s now going unfunded, student opportunities being rescinded, and lab work grinding to a halt due to uncertainty and inconsistent support. Dr. Caldwell discusses the personal sacrifices needed to keep research and student training alive, the hidden infrastructure supporting breakthroughs, and the transformative power of investing in the next generation. Discover why basic and translational research are in jeopardy, how rare disease investigations can yield major insights, and what must change for the U.S. to remain a global science leader.

This in-depth discussion is essential for anyone concerned about public health, America’s future competitiveness, and the invaluable role of federal science funding. Explore what’s truly at stake—from lab experiments to lawmakers’ decisions—and learn how viewers can add their voices to support urgent, sustained investment in research that touches every family.

Join us for an exciting deep dive with Isaac Plutzer, a graduate researcher at Washington University in St. Louis, as he explores the mysterious "dark kinome"—the hundreds of under-studied protein kinases with high potential for therapeutic discovery. In this interview, Isaac explains what kinases are and why a substantial fraction remain enigmatic, despite being critical regulators of cellular processes.

Isaac shares how his lab combines innovative proximity labeling and phosphoproteomics techniques with advanced mass spectrometry to systematically map the interactors, substrates, and signaling pathways of these mysterious enzymes. The conversation covers:

• What makes a kinase “dark” and why it matters for both basic biology and drug development

• How proximity labeling and mass spectrometry workflows (including TMT-based and DIA-based approaches) are expanding our map of protein interactions

• The unique challenges of complex data analysis—including imputation of missing values—and the evolving computational landscape

• Translational opportunities for new kinase-targeting drugs, and a look at the cutting edge of single-cell proteomics and automation

• Personal reflections on scientific discovery, experimental setbacks, and advice for young scientists entering the rapidly growing fields of proteomics and systems biology

Whether you’re passionate about biochemistry, analytical technology, or excited by scientific frontiers, this interview offers approachable insights into how next-generation tools are rewriting our understanding of the cell.

Listen in and be inspired by the thrill of the unknown, the promise of mass spectrometry, and the possibilities for the future of biomedical research!

In this special episode of Concentrating on Chromatography, host David Oliva steps into the guest role to share his journey as featured on the Lab Matters podcast. David discusses his transition from a passion for business and economics to leading Organomation, a renowned manufacturer of laboratory instrumentation and sample preparation tools. Organomation's flagship products—like the N-EVAP, MULTIVAP, and MICROVAP nitrogen evaporators—help scientists accelerate the sample concentration process. These instruments utilize gentle nitrogen gas flow and precise temperature control, enabling labs to prepare sensitive samples with accuracy and efficiency. David explains how these technologies are trusted by academic, government, and commercial laboratories worldwide, including leading analytical instrument manufacturers.

Listeners will gain insight into how learning agility and transferable skills drive success across industries, and why David is devoted to making scientific workflows more sustainable and less wasteful. Explore the practical impact of Organomation’s solutions—from facilitating complex analyses to reducing environmental impact—and discover what it takes to lead innovation in sample preparation. If you care about chromatography, scientific careers, or the future of lab technology, this episode is essential listening. Find us at ConcentratingOnChromatography.com or on LinkedIn.

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.In this episode, you'll learn:- How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.- The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.- Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.- Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.- Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.Referenced Papers:- "Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)- "New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)- "Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.Subscribe for more interviews with pioneering scientists in chemistry, biology, and biomedical research!#MalariaResearch #StructuralBiology #DrugDiscovery #KinaseInhibitors #Proteomics #Chromatography #MassSpectrometry #Falcipain2 #Antimalarial #ScientificCareers #UCFResearch

In this episode of the Concentrating on Chromatography podcast, host David Oliva interviews Ainslie Chen about her cutting-edge research on hemoglobin variant testing using liquid chromatography-high resolution mass spectrometry (LC-HR-MS). Ainslie discusses her novel method for separating and identifying hemoglobin subunits with mass shifts less than 1 Da, overcoming critical limitations in conventional clinical assays.Hear firsthand how her team developed an LC-HR-MS workflow using a C4 reversed-phase column, tackled technical challenges (including why C18 columns fell short), and achieved unprecedented sensitivity for variant detection in both adult and neonatal samples. The conversation touches on the potential of mass spectrometry to revolutionize clinical diagnostics, the importance of reproducibility, barriers to adoption in routine labs, and how teamwork drives scientific innovation.Perfect for analytical chemists, clinical laboratorians, and anyone interested in the future of protein variant analysis, this episode also features Ainslie’s advice for early-career scientists and insights from her first ACS poster presentation.Key Highlights:- Motivation behind improving hemoglobinopathy testing - Technical advantages of LC-HR-MS over gene sequencing and CE - The crucial role of the C4 column in resolving challenging samples - Critical parameters for reproducible LC-HR-MS workflows - Barriers and outlook for clinical adoption of advanced mass spectrometry - The value of teamwork and mentorship in scientific research - Direct insights from recent ACS conference experiencesIf you’re curious about where analytical chemistry meets clinical medicine and the innovations shaping the next generation of diagnostic tools, don’t miss this illuminating interview!#LCHRMS #HemoglobinVariants #AnalyticalChemistry #ClinicalDiagnostics #Chromatography #MassSpectrometry

Join us for an insightful conversation with Pernilla Sörme, Technical Program Director at My Green Lab, as we explore how one organization is transforming laboratories worldwide into leaders in environmental sustainability. Discover the driving mission behind My Green Lab, the measurable impact on research and industry, and real-world tips for saving energy and reducing plastic waste in scientific labs. Pernilla shares how behavior change, grassroots and leadership buy-in, and a community-driven approach are helping thousands of labs certify their sustainability practices—and why this matters for the future of science, the planet, and organizational success. Whether you’re in academic research or commercial R&D, this candid interview explains why sustainability, reputation, and cost savings can go hand-in-hand through My Green Lab Certification, ACT Eco-label, and more. Watch for practical advice, industry milestones, and a vision for science that benefits people and the planet.

Join analytical chemist Dr. M. Farooq Wahab from the University of Texas at Arlington as he breaks down the science behind chromatographic peak shapes in this episode of the Concentrating on Chromatography podcast. Perfect for undergraduate chemists and researchers alike, this conversation covers:- What makes an ideal chromatographic peak shape and why it matters - Common reasons your HPLC peaks might show tailing, fronting, or distortion - Practical tips for diagnosing and troubleshooting peak shape problems - How to use visual and digital tools (like Excel) to analyze your chromatogram data beyond basic numbers - The importance of baseline correction, denoising, and signal processing in chromatography - Advice for students and early researchers aiming to excel in analytical chemistryDr. Wahab shares lively insights from his research and decades of experience, revealing the underlying physics, chemistry, and engineering behind the peaks you see—and why peaks tell the story of your chromatography.🔬 Whether new to HPLC or looking to deepen your understanding, this episode demystifies a critical topic that impacts every chromatographer.Don’t forget to like, subscribe, and ring the bell for more expert chromatography content! Subscribe here: https://www.youtube.com/@UCU9oYSK_peqfuCrwpnv4fqA HPLC peak shapes, chromatography troubleshooting, peak tailing, peak fronting, chromatographic peak analysis, analytical chemistry podcast, undergrad chromatography, baseline correction HPLC, signal processing chromatography, Farooq Wahab, Concentrating on Chromatography, chromatography tips, peak shape Gaussian, chromatography data analysis

Join us in this fascinating episode of Concentrating on Chromatography as Dr. Rachael Guenter, PhD, shares her eight-year journey researching neuroendocrine tumors. Discover how her background in plant pathology and engineering shaped her translational medicine approach, and learn about the crucial role of mass spectrometry in uncovering protein-level insights in these rare cancers. Dr. Guenter also discusses the challenges of translating lab discoveries into clinical trials, the promise of spatial mass spectrometry for tumor heterogeneity, and the importance of collaboration between scientists, clinicians, and patients. Whether you're interested in cancer biology, mass spec applications, or cutting-edge cancer research, this episode delivers valuable perspectives and inspiring advice from a leading expert in the field. Subscribe for in-depth scientific conversations and cutting-edge chromatography content every week. #NeuroendocrineCancer #MassSpectrometry #CancerResearch #ChromatographyPodcast #TranslationalResearch #SpatialMassSpec #DrRachaelGuenter

Join us for an insightful conversation with Dr. Ryland Giebelhaus a chromatographer and metabolomics researcher from the University of Victoria, as he breaks down fecal microbiota transplantation (FMT)—a revolutionary treatment changing lives by restoring healthy gut bacteria. In this episode, you’ll learn:- What FMT is and why it’s a game-changer for treating recurrent Clostridioides difficile infections and other gut-related diseases - How cutting-edge techniques like comprehensive two-dimensional gas chromatography (GC×GC-TOFMS) enable detection of key metabolites such as short-chain fatty acids, linking gut microbiome function to health - Practical challenges of analyzing biological samples, including sample prep and maintaining bacterial viability for research and clinical use - The role of advanced chromatography and mass spectrometry in monitoring FMT product stability, efficacy, and future therapeutic design - Why data science and coding skills are essential for next-generation metabolomics and microbiome research - The exciting future of multidimensional separations (GC×GC, LC×LC) in expanding our understanding of complex biological systems Whether you’re an undergraduate entering analytical chemistry or a researcher curious about the intersection of microbiology and metabolomics, this episode offers valuable perspectives on innovative science improving patient health.🔬 Don’t forget to like, subscribe, and click the bell for more expert interviews and breakthroughs in chromatography, mass spectrometry, and analytical sciences.#FMT #FecalMicrobiotaTransplant #Metabolomics #GCxGC #Chromatography #GutHealth #MicrobiomeResearch #MassSpectrometry #AnalyticalChemistry #Microbiota

Join host David Oliva from Organomation for an in-depth conversation with Sean Orlowicz, Principal Marketing Development Manager at Phenomenex, about the analytical challenges and innovative solutions for GLP-1 peptide therapeutics like semaglutide and tirzepatide.In this episode, presented in collaboration with Separation Science (https://www.sepscience.com/)—a premier learning platform for analytical scientists—Sean shares over 22 years of chromatography expertise covering critical topics including:Key Discussion Topics:- Unique analytical challenges in separating GLP-1 analogues and related impurities- Column selectivity and efficiency optimization for complex peptide separations- The role of Aeris Peptide XB-C18 and core-shell particle technology- Method robustness, transferability, and regulatory considerations for sterile injectables- Size exclusion chromatography for aggregate analysis- High-resolution mass spectrometry applications in peptide analysis- Preparative chromatography for API purification at commercial scale- Common pitfalls and troubleshooting tips for peptide method development- The genericization of GLP-1 therapeutics and global market trendsAbout the Guest:Sean Orlowicz brings extensive experience from both laboratory and global pharmaceutical market perspectives at Phenomenex, specializing in peptide analysis workflows from API manufacturing to analytical testing.This episode is brought to you in collaboration with Separation Science, the leading online educational platform providing chromatographers with expert tutorials, webinars, application notes, and learning modules across LC, GC, MS, and separation technologies.Special thanks to Phenomenex for their continued innovation in developing chromatography solutions that advance pharmaceutical analysis, including specialized columns and methods for GLP-1 peptide therapeutics.Resources Mentioned:- Phenomenex GLP-1 Applications Notebook- Biozen dSEC-1 SEC columns for aggregate analysis- Technical notes on semaglutide and tirzepatide analysis- Visit www.phenomenex.com for application support- Explore www.sepscience.com for chromatography education and trainingRelevant for: Analytical chemists, pharmaceutical scientists, method development specialists, HPLC/UHPLC users, peptide researchers, quality control professionals, and separation scientists working in biopharma, generic pharmaceuticals, and regulatory environments.#Chromatography #GLP1 #PeptideAnalysis #HPLC #Semaglutide #Phenomenex #SeparationScience #AnalyticalChemistry #MethodDevelopment #Pharmaceuticals

In this episode of Concentrating on Chromatography, David interviews his former high school chemistry teacher, Dr. Kristen Vanderveen from The Bromfield School, about teaching separation science at the high school level.Dr. Vanderveen shares her approach to introducing chromatography, filtration, and gravimetric analysis to both first-year chemistry students and AP Chemistry students. She discusses the hands-on experiments that work best in a high school setting, including paper chromatography with food dyes, TLC separations, and the challenges of balancing lab time with curriculum requirements.Topics discussed:- Separation methods taught in high school chemistry (filtration, paper chromatography, TLC)- Gravimetric analysis and precipitation experiments for AP Chemistry- Student engagement strategies and memorable lab activities- Balancing theoretical concepts with hands-on experimentation- Common student misconceptions about separation techniques- Preparing students for college-level chemistry coursesFollow Dr. Vanderveen's chemistry education content:YouTube: https://www.youtube.com/@drvchemistryAbout the Guest:Dr. Kristen Vanderveen is a chemistry teacher at The Bromfield School with over 20 years of experience teaching high school chemistry, including AP Chemistry. Her background includes working as a protein chemist before transitioning to education.About Concentrating on Chromatography:This podcast series explores chromatography and analytical chemistry through conversations with scientists, educators, and industry professionals.#Chromatography #ChemistryEducation #SeparationScience #HighSchoolChemistry #APChemistry #STEMEducation #AnalyticalChemistry #ScienceTeaching

In this episode of Concentrating on Chromatography, we sit down with Andreas Metousis, a PhD researcher at the Max Planck Institute of Biochemistry, to explore cutting-edge spatial proteomics and its role in understanding ovarian cancer development.Andreas discusses how Deep Visual Proteomics (DVP)—a method combining artificial intelligence, laser micro-dissection, and advanced mass spectrometry—is revolutionizing cancer research by providing unprecedented insight into the earliest molecular events in disease progression.Key Topics Covered:- How transcriptomics and proteomics differ and why both matter for cancer research- Deep Visual Proteomics (DVP): AI-driven cell identification and high-resolution protein analysis- Why high-throughput automation (384-well plates, EVOSEP, Thermo Fisher Orbital Astral Mass Spectrometer) is essential for modern proteomics- The IDO1 paradox: why an "immune evasion" protein actually protects cancer cells—and why that matters for failed clinical trials- Translating lab discoveries into real-world therapeutics: the drug development pipeline- Applying spatial proteomics beyond ovarian cancer (lung cancer, skin cancer, osteoarthritis, muscle biology)- Advice for students entering cancer research and academiaWhy This Matters:Andreas's work demonstrates how multi-modal omics integration and spatial resolution can identify novel drug targets and explain why some promising therapies fail in clinical practice. This episode bridges fundamental science with practical lab methodology and therapeutic impact.Perfect for: Analytical chemistry professionals, cancer researchers, graduate students, and anyone interested in how cutting-edge mass spectrometry and AI are transforming biomedical research.Spatial Proteomics, Mass Spectrometry, Ovarian Cancer, Deep Learning, AI in Science, Proteomics, Transcriptomics, Cancer Research, Drug Discovery, EVOSEP, Thermo Fisher Orbital Astral Mass Spectrometer, Laser Microdissection, IDO1, Immunotherapy, Analytical Chemistry

In this episode of Concentrating on Chromatography, we sit down with Alex Brody, an organic chemist at Aqua America, to discuss the rigorous analytical methods required for regulated drinking water testing.Alex walks us through the multi-step PFAS detection process using EPA Method 537.1, including extraction, nitrogen blowdown concentration, and LC-MS analysis. He explains why sample preparation and quality control are critical for achieving trace-level detection—and why these methods can't be rushed or simplified, even with new technologies available.We also explore taste and odor investigations, disinfection byproducts, and volatile organic compounds, plus the surprising reality of why regulated labs move slowly when adopting new instrumentation. You'll learn about the quality control checkpoints, peer review processes, and the misconceptions surrounding analytical turnaround times in compliance labs.Topics Covered:- Role of organic chemistry in regulated drinking water labs- EPA compliance requirements and regulatory bodies (EPA, PA-DEP)- PFAS detection using Method 537.1 and LC-MS- The critical importance of nitrogen blowdown sample concentration- Taste and odor analysis: MIB, Geosmin, and unknown compound identification- Why regulated methods evolve slowly (validation timelines, approval processes)- Quality control procedures: calibration checks, matrix spikes, surrogate standards, internal standards- Automated sample preparation and lab efficiency- Future PFAS regulations and Method 533 expansionIdeal For:- Analytical chemists and environmental lab professionals- Water utilities and compliance officers- Chromatography practitioners interested in regulated workflows- Anyone curious about how drinking water safety is ensured

Join us for an in-depth conversation with Holly Lee, Global Technical Marketing Specialist at SCIEX, as we explore the cutting edge of food and environmental safety testing. Holly shares her expertise on analyzing ultra-short chain PFAS like TFA, masked mycotoxins, pesticide residues, and the latest LC-MS/MS workflows transforming laboratory efficiency.In this episode of Concentrating on Chromatography, we discuss:PFAS Analysis & Challenges- Why ultra-short chain PFAS compounds like trifluoroacetic acid (TFA) are among the toughest analytes to detect- Background contamination issues and how labs can minimize them- Column selection strategies and method development for comprehensive PFAS panels- Combining different stationary phase chemistries to broaden selectivityMycotoxin Detection- What are masked mycotoxins and why they evade conventional detection- Climate-driven changes in mycotoxin co-occurrence patterns- Achieving ultra-trace sensitivity for regulated limits in food matrices- EU regulations vs. global standards for mycotoxin testingFood Residue Testing Innovations- Multi-residue pesticide methods covering 100+ compounds- Overcoming challenges with complex food matrices like tea, juice, and extracts- Veterinary drug residue analysis and validation strategies- The role of high-resolution mass spectrometry (HRMS) in resolving matrix interferencesLaboratory Workflow Optimization- Sample preparation strategies: when to use direct injection vs. SPE cleanup- Automation, AI, and machine learning for peak integration and data processing- Green chemistry practices and sustainability in food testing- Getting the most from your LC-MS/MS software featuresHolly's Background & Insights- Journey from studying PFAS fate at University of Toronto to food safety applications- Experience spanning Ontario Ministry of Environment, SCIEX R&D, and global technical marketing- Emerging contaminants on the horizon, including microplastics analysis with LC-MS/MS- Partnership between SCIEX and Phenomenex for advancing chromatography solutionsResources Mentioned:- NIST PFAS Interference List (PIL) database- Phenomenex Luna Omega PSC18 column for ultra-short chain PFAS- SCIEX 7500 and 7600 ZenoTOF systems- Science of the Total Environment journal article on microplasticsWhether you're a food safety analyst, environmental chemist, or chromatography enthusiast, this conversation offers practical insights into method development, troubleshooting, and the future of analytical testing.🔬 About the Guest:Holly Lee is a Global Technical Marketing Specialist for Food Applications at SCIEX with a PhD from the University of Toronto studying PFAS environmental processes. She has hands-on experience in analytical method development, LC-MS/MS analysis, and worked at the Ontario Ministry of the Environment before joining SCIEX.🎙️ About Concentrating on Chromatography:A podcast exploring the science, applications, and innovations in chromatography and sample preparation for analytical laboratories worldwide.Hashtags:#Chromatography #LCMSMS #FoodSafety #PFAS #Mycotoxins #AnalyticalChemistry #LabTesting #SCIEX #EnvironmentalTesting #PesticideResidue #TFA #MaskedMycotoxins #LabAutomation #FoodAnalysis #SamplePreparation

Connor Johnson, a researcher from the University of Alberta, discusses his award-winning honours project analyzing the volatile organic compounds (VOCs) in two banana species using headspace gas chromatography-mass spectrometry (HS-GC-MS). He completed this specific project as an undergraduate at Thompson Rivers University (TRU).For over 60 years, commercial banana flavoring has remained unchanged—even though the fruit it's supposed to mimic changed in the 1950s. Connor's research reveals why fake banana tastes fake: the commercial banana extract contains only 3 compounds compared to 18+ in real bananas, missing critical compounds that create authentic banana flavor.This episode covers:- The history of banana flavoring and the myth of the Gros Michel banana- What Connor discovered when comparing Cavendish vs. Gros Michel bananas- The real compounds behind authentic banana flavor (hint: it's not just isoamyl acetate)- Why headspace GC is ideal for volatile organic compound analysis- Challenges with sample prep and instrument troubleshooting in research- How this research could revolutionize flavor chemistry in the food industry- The broader applications of comparing artificial flavorings to real fruitsConnor won two national conference awards for this work and shares insights into the analytical challenges of flavor chemistry, including instrument downtime, sample matrix effects, and why creating authentic synthetic flavoring is harder than it seems.Perfect for chemistry students, flavor scientists, and anyone curious about why banana candy tastes nothing like real bananas.

In this episode of Concentrating on Chromatography, we sit down with Dr. Lee Polite from Axion Training Institute to break down one of analytical chemistry's most powerful yet misunderstood techniques: gas chromatography-mass spectrometry (GC/MS).What You'll Learn:- Why GC and MS are the "perfect pair" – and what happens when you try to use MS alone- The electron gun: how molecules get ionized and why they become positively charged (not negatively!)- The magnetic sector vs. quadrupole: from first principles to modern mass filtering- Why Dr. Lee uses the "corkscrew trajectory" analogy – and why it actually works- The cars and boats analogy: how fragmentation creates a unique molecular fingerprint- Scan mode vs. SIM (Selected Ion Monitoring): when to use each for identification vs. sensitivity- Real-world forensics: detecting pesticides in spinach and cocaine in hair follicles- Triple quad GC/MS and Multiple Reaction Monitoring (MRM): the future of trace analysisWhy This Matters:Over 2 million chromatographs operate worldwide, yet most users don't truly understand how they work. Dr. Polite has trained more than 14,000 professional scientists at Axion Labs to move beyond "pushing buttons" to genuinely comprehending the science. This conversation is designed for undergraduate students, academic researchers, and anyone preparing for analytical chemistry roles in pharma, environmental testing, or forensics.The Teaching Philosophy:Dr. Polite breaks complex instrumentation into simple, transferable concepts. He uses real analogies (shopping malls, bank robberies, and magnetic levitation) to make abstract physics tangible. By the end of this episode, you'll understand that mass spectrometry isn't magic—it's elegant physics made practical.Guest Information:Dr. Lee Polite is a leading authority in analytical chromatography education and founder of Axion Training Institute, a real working laboratory where scientists come for hands-on GC and LC training. With nearly 30 years of experience and a PhD under Harold McNair (one of the grandfathers of modern chromatography), Dr. Polite is passionate about making complex instrumentation accessible to students and professionals alike.Resources & Links:🔗 Axion Training Institute: www.chromatographytraining.com🔗 Email: info@axionlabs.com📧 Subscribe to Concentrating on Chromatography for more expert interviews on analytical separation science#MassSpectrometry #AnalyticalChemistry #GCMSAnalysis #ChromatographyEducation #LabInstrumentation #Chemistry #SeparationScience #Quadrupole #Instrumentation #UndergraduateChemistry

Join us as we sit down with Jim Gearing, Associate Vice President of Marketing for Agilent's Gas Phase Division, to explore how the world of chromatography is changing and how intelligent instrument design is meeting users where they are.This interview was conducted in collaboration with Separation Science, the premier online learning platform for analytical scientists, providing expert content on chromatography, mass spectrometry, sample preparation, and related laboratory techniques.In this episode, Jim shares insights from 34 years at Hewlett Packard/Agilent—including 22 years in R&D—on three critical shifts reshaping analytical labs:🔬 Changing Demographics of Users- How the lab workforce is evolving: fewer experienced analysts, higher turnover, less formal training- Why modern users expect instruments to work like the consumer tech in their hands (iPhones, tablets, apps)- Real-world stories from labs operating with skeleton crews—managing entire instrument rooms with 1–2 people🎯 User-Input-Guided Design- How Agilent collects feedback from day one: customer site visits, service teams, quality data, and early-stage prototyping- The evolution from paper flipcharts (1990s) to rapid software prototyping and eye-tracking today- Concrete examples of pain points that drove major design changes (easy maintenance, remote data access, intelligent diagnostics)🤖 Intelligent Instrument Systems- What "intelligent" really means: features that remove workload and mental effort while delivering high-confidence results- Built-in capabilities like peak evaluation, retention time locking, and maintenance wizards that prevent errors before they happen- How smart instruments operate independently—**they don't require internet connectivity** (addressing a key misconception)- Why distributed intelligence (in the instrument, software, and enterprise services) gives labs flexibilityPlus:- Advice for lab directors building long-term instrumentation strategies (goals, users, solutions)- Jim's magic wand fix: eliminating time spent on non-value-added data processing- Why analytical instrumentation matters beyond the lab—safer food, cleaner water, better pharmaceuticalsPerfect for:- Early-career chromatographers and analytical chemists- Lab managers and directors evaluating instrumentation strategy- Anyone curious about how intelligent systems are reshaping laboratory workflowsGC-MS, chromatography, intelligent instruments, lab automation, user experience, analytical chemistry, Agilent, instrument design, laboratory instrumentation, workflow optimization, data processing, lab management, training and onboarding

In this episode, we interview Daniel Reddy, 2025 CAS Future Leader and PhD candidate at Queen's University, about his groundbreaking research on automated mass spectrometry and dried matrix spots (DMS).Dan's work combines computer vision, 3D printer automation, and laser micromachining to revolutionize sample preparation—reducing CO₂ emissions by 28-fold and organic solvent use by 21-fold compared to traditional methods.What You'll Learn:How to give a mass spectrometer "sight" and "taste" using computer vision and the LMJ-SSP (Liquid Microjunction Surface Sampling Probe)The breakthrough technology behind Surface Energy Traps (SETs) for confining liquid droplets on paper substratesWhy dried matrix spots eliminate the need for cold-chain shipping and enable analysis of blood, urine, and saliva samples via standard mailHow DIY chemists are hacking 3D printers to build cost-effective autosamplers (replacing $10K+ systems)The role of green chemistry and systems thinking in modernizing analytical methodsWhy interdisciplinary collaboration (chemistry + computer science) is critical to innovationKey Topics:Dried Matrix Spots (DMS) for automated sample prepLaser-micromachined Surface Energy TrapsDirect surface sampling mass spectrometrySustainability in analytical chemistry3D printer customization for laboratory automationThe importance of science communication and community outreachGuest Background:Dan Reddy is a PhD candidate in the Department of Chemistry at Queen's University and a recipient of the NSERC Vanier Canada Graduate Scholarship. He was recently named one of the top 35 early-career scientists globally in the 2025 CAS Future Leaders program.Relevant for:Analytical chemists and mass spectrometry practitionersLab managers seeking sustainable and cost-effective sample prep solutionsResearchers interested in green chemistry and automationDIY enthusiasts and makers interested in laboratory innovationStudents pursuing careers in analytical chemistry

How do the hidden carbohydrate structures on your favorite protein powders shape the gut microbiome? In this episode of Concentrating on Chromatography, Matthew Bolino, M.S., from the University of Nevada, Reno, breaks down his latest research on N‑glycans from common dietary proteins (whey, egg white, soy, and pea) and how their structural diversity influences microbial fermentation and short‑chain fatty acid production.Bolino explains what N‑glycans are, why they behave like fiber in the gut, and how his team isolates and characterizes them using ethanol washes, enzymatic release (PNGase F and gut‑derived endoglycosidases), and advanced MALDI‑TOF and HILIC‑QTOF workflows. He also discusses his 2025 work comparing synthetic versus bovine whey N‑glycomes and mapping N‑glycan profiles across dietary protein sources, revealing how glycan architecture can reshape community diversity in in vitro fecal fermentations.Geared toward undergraduate and early‑career analytical chemists, this conversation dives into practical mass spec trade‑offs (MALDI vs QTOF vs LC/GC), real‑world troubleshooting in glycomics labs, and how microbiome‑targeted therapeutics and “symbiotic” designs may emerge from pairing specific microbes with preferred glycan structures. Bolino closes with career advice on building biomolecular analysis skills, understanding instrumentation fundamentals, and entering the rapidly growing field of glycomics and microbiome research.

In this episode of Concentrating on Chromatography, host David Oliva sits down with Dr. Steven Weinman, Associate Professor of Chemical and Biological Engineering at the University of Alabama, to explore how membrane science and chromatography intersect in modern separation challenges.Steven shares his journey from chemical engineering student to membrane researcher, and explains how membranes are used not only for water purification, but also for sample preparation, pre-treatment, and concentration in analytical workflows. The conversation dives deep into PFAS removal, nanofiltration vs. reverse osmosis, and how chromatography and mass spectrometry are essential for validating membrane performance.Key topics discussed include:* How membranes function as separation and concentration tools* Nanofiltration vs. reverse osmosis for salts and PFAS* The role of chromatography (LC-MS, GC-MS, ion chromatography) in verifying contaminant removal* Challenges in scaling academic separation technologies to industry* Sustainability in membrane manufacturing and PFAS-related regulations* Training students to balance fundamental science with real-world applicationsWhether you work in environmental analysis, chromatography, mass spectrometry, water quality, or separation science, this episode provides valuable insight into how different separation technologies complement each other—and where the field is heading next.🎧 Subscribe for more conversations on chromatography, sample preparation, and analytical science.

Haloacetic acids (HAAs) are disinfection byproducts formed when chlorine or bromine reacts with organic matter in water—and some are linked to serious health concerns. In this episode of Concentrating on Chromatography, we sit down with Jessica Whitehouse, MSc student at the University of Calgary, to discuss how she developed a GC-MS method to detect and quantify HAAs in real-world water samples.During her undergraduate research at Thompson Rivers University, Jessica tackled a major challenge faced by many academic labs: how to analyze regulated environmental contaminants without access to GC-ECD instrumentation. Using dispersive liquid–liquid microextraction, derivatization, and GC-MS, she built a faster, more accessible workflow—and applied it to tap water, swimming pools, and hot tubs.In this conversation, we cover:* What haloacetic acids are and why they matter* Why standard EPA methods can be difficult for smaller or teaching-focused labs* How GC-MS can be adapted for HAA analysis* The challenges of derivatization and temperature program optimization* Unexpected findings in brominated vs. chlorinated HAAs* Why pool and hot tub water can show surprisingly high HAA levels* The excitement (and frustration) of first-time method development* Advice for undergraduate and early-career analytical chemistsJessica also shares how this project led directly to her current MSc research on ozone and nanobubble water disinfection, where she’s now expanding into ion chromatography.Whether you work in **environmental analysis, chromatography, GC-MS, or are just starting your journey in analytical chemistry**, this episode offers practical insight into real lab constraints, method development, and the joy of finding your first analyte peak.🔬 Topics: GC-MS, haloacetic acids, water analysis, method development, derivatization, environmental chemistry🎓 Audience: Academic researchers, students, environmental labs, analytical chemists

Neglected and opportunistic infectious diseases affect some of the world’s most vulnerable populations—but often receive the least attention from traditional drug discovery pipelines.In this episode of Concentrating on Chromatography, host David Oliva sits down with Brad Haubrich to explore how early-stage drug discovery is being applied to fungal and parasitic pathogens, including those responsible for neglected tropical diseases and infections that disproportionately affect immunocompromised patients.Brad shares how his lab approaches drug discovery when the pathogen is eukaryotic—and therefore biologically similar to humans—making selectivity one of the biggest challenges. The conversation covers:* What defines neglected and opportunistic diseases—and why commercial incentives often fall short* Target-based vs. phenotypic drug discovery and when each approach makes sense* Using binding kinetics and residence time to improve selectivity and reduce off-target effects* Where chromatography, metabolomics, and mass spectrometry still play a critical role—even when not front-and-center* The growing (and realistic) role of AI in drug discovery, especially for underfunded disease areas* Why World Neglected Tropical Diseases Day (January 30) matters for raising awareness and accelerating progressThis episode highlights how analytical chemistry, separation science, and biological insight intersect in the earliest stages of drug discovery—and why progress in this space depends as much on collaboration and curiosity as it does on technology.🎙️ **Recorded in recognition of World Neglected Tropical Diseases Day**

How do chemists design molecules that safely carry radioactive metals through the body to target cancer cells?

In this episode of Concentrating on Chromatography, David sits down with Simona Mastroianni and Marianna Tosato to explore the chemistry behind radiopharmaceuticals — drugs that combine radioactive isotopes with specially designed chelators to diagnose and treat cancer.

Their latest research focuses on the theranostic pair lead-203 and lead-212, a powerful combination that enables both imaging and targeted alpha therapy using the same chemical platform. To make this possible, they developed new “molecular cages” that tightly bind lead ions, improving stability, safety, and effectiveness in the body.

Along the way, we break down:

• What radiopharmaceuticals and theranostics actually mean

• Why chelators act like cages for radioactive metals

• How chromatography (HPLC/TLC) verifies radiolabeling and purity

• How NMR shows metals are truly bound

• The path from synthetic chemistry → animal studies → hospitals

• Career advice for undergraduate chemists interested in medical and radiochemistry

If you’ve ever wondered how analytical chemistry, inorganic chemistry, and separation science translate into real cancer treatments, this episode connects the dots.Based on their recent publication demonstrating highly stable, efficiently labeled cyclen-based chelators for 203/212Pb radiopharmaceuticals and the full interview discussion .🎧

Perfect for students in:Analytical chemistry • Chromatography • Inorganic chemistry • Radiochemistry • Pharmaceutical sciences

radiopharmaceuticals, lead-212 therapy, theranostics, chelators, chromatography, HPLC, NMR, radiochemistry, cancer drug development, analytical chemistry careers

How do you see proteins, metals, and disease processes inside real tissue — and still trust the numbers?In this episode of Concentrating on Chromatography, David sits down with Monique Mello, analytical chemist, educator, and LA-ICP-MS imaging specialist, to explore how laser ablation ICP-MS (LA-ICP-MS) and immuno-mass spectrometry imaging (iMSI) are transforming pathology, environmental science, and translational research.Monique shares her journey from public-health and pathology labs in Brazil to environmental and biomedical research in Australia — and explains why metrology, traceability, and defensible measurements are the foundation of meaningful science.We dive into her work developing multiplexed elemental imaging methods that allow researchers to quantify multiple proteins at once in tissue — revealing interactions that traditional single-marker methods miss. Her studies show how LA-ICP-MS can map dystrophin-glycoprotein complex proteins in muscular dystrophy and track elemental distributions like zinc in Alzheimer’s disease tissue.We also discuss something many labs overlook: sample preparation and immunolabelling can change the chemistry you’re trying to measure. Monique’s research demonstrates how staining steps can redistribute endogenous metals and why rigorous validation is critical for trustworthy data.If you care about chromatography, mass spectrometry, or analytical chemistry that genuinely impacts patients and communities, this episode is for you.In this conversation, we cover:• What LA-ICP-MS imaging is and how it works• Multiplexed antibody tagging with lanthanides for quantitative tissue imaging• Why metrology and uncertainty matter more than “pretty data”• Common analytical failures (and why sample prep causes most of them)• Elemental mapping in muscular dystrophy and Alzheimer’s research• How immunolabelling and coverslipping can perturb endogenous metals• Teaching analytical chemistry for real-world problem solvingWho this is for: Analytical chemists • Mass spectrometrists • Chromatographers • Pathology researchers • Environmental scientists • Students entering the field

In this episode of Concentrating on Chromatography, David speaks with Francis Femi Oloy about using chromatography to uncover hidden pollutants in real-world water systems.Femi’s team analyzed polychlorinated biphenyls (PCBs) in six major rivers in southwestern Nigeria — compounds that were banned decades ago but still persist in the environment. Using a workflow that many analytical labs will recognize — liquid–liquid extraction, cleanup, rotary evaporation, nitrogen blowdown, and GC-ECD detection — they quantified 25 PCB congeners at trace levels and linked the results to ecological and human health risk.📌 In this conversation, we cover:• Why legacy pollutants like PCBs still show up today• Choosing GC-ECD vs LC-MS for halogenated compounds• Liquid–liquid extraction and matrix cleanup strategies• Why sample concentration is critical for dilute environmental samples• How rotovap + nitrogen blowdown work together without losing volatile analytes• Seasonal trends (why wet season levels were higher)• Translating concentration data into meaningful risk assessmentsThis episode is perfect for anyone working in:Chromatography • Environmental analysis • Sample prep • Trace analysis • GC methods • Analytical chemistryIf you enjoy practical discussions about real laboratory workflows and how chromatography solves real problems, subscribe to Concentrating on Chromatography. 🔬 Paper discussed: Polychlorinated biphenyls (PCBs) in rivers of Southwestern Nigeria: sources, seasonal distribution, and assessment of human health risks# 🔔 More episodesSubscribe for more interviews with scientists using chromatography and mass spectrometry to solve real-world challenges.

In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.Drawing from her ACS Northeast presentation and this in-depth conversation, Lindsay explains:🔬 How photoredox catalysts become activated under visible light📊 Why LC-MS was essential when NMR couldn’t resolve complex mixtures📈 How to design reproducible calibration curves for percent catalyst remaining📉 Why extracted ion chromatograms (EIC) outperform total ion chromatograms (TIC) at low concentrations⚗️ How solvent activation chemistry led to selective N-demethylation🧪 Why GC-MS with an internal standard streamlined reaction screening📐 What relative response factors mean — and why they can’t always be assumed constant🧑‍🔬 Practical tips for improving reproducibility (microbalances, deoxygenated solvents, temperature control)This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.If you work in:* Photoredox chemistry* Reaction optimization* Mass spectrometry method development* Catalyst screening* Academic synthetic chemistry…this conversation will resonate.🧪 Key Topics Covered* Photocatalyst stability in DMF, DCE, and MeCN* Demethylation under mild visible-light conditions* High-resolution Q-TOF LC-MS quantitation* Internal standard methodology in GC-MS* Signal-to-noise improvement using extracted ion chromatograms* Reaction reproducibility and quality control strategy🎙 About the GuestLindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.If you enjoy conversations at the intersection of chromatography and real-world chemistry research:👍 Like💬 Comment with your LC-MS / GC-MS questions🔔 Subscribe for more episodes of

Light can heal. Light can power devices. But light can also destroy molecules.In this episode of Concentrating on Chromatography, we sit down with Kshmeya Chopra to explore how phthalocyanines — highly conjugated macrocycles used in photodynamic therapy, sensing, and organic electronics — respond to prolonged light exposure.Using UV–Vis spectroscopy, Kshmeya and her research team systematically investigated how:• The central metal (Zn²⁺ vs In³⁺)• Degree of fluorination• Axial ligands• Solvent environment (EtOAc vs DMSO)influence photostability under two-sun irradiation conditions.By monitoring changes in the Q-band absorbance over time and calculating extinction coefficients using Beer–Lambert law, the team uncovered clear structure–property relationships governing light-induced degradation.We discuss:🔬 How UV–Vis spectroscopy tracks molecular breakdown🧪 Aggregation vs true chemical degradation☀️ Why fluorination improves photostability⚖️ Zinc vs indium coordination effects📊 Extinction coefficients and what they reveal about macrocycle behavior🧬 How LC–MS and HRMS could identify degradation products🎓 Advice for undergraduate students entering photochemistry and analytical researchThis conversation bridges spectroscopy, materials chemistry, and analytical science — showing how subtle molecular design choices dramatically impact stability and real-world application potential.If you’re interested in photochemistry, UV–Vis analysis, chromatography, or rational molecular design, this episode is for you.🎙️  @ChromatographyTalk  explores the intersection of analytical chemistry, instrumentation, and applied molecular science.Subscribe for more conversations on LC-MS, GC-MS, spectroscopy, and chemical problem-solving.#spectroscopy

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#Fluorination

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Liquid chromatography–mass spectrometry (LC-MS) has been the backbone of modern analytical workflows for decades — but what if one of its most trusted components is also its biggest bottleneck?In Episode 50 of Concentrating on Chromatography, host Dave Oliva sits down with Daniel DeBord, Chief Technology Officer at MOBILion Systems, to explore how high-resolution ion mobility may be changing the way scientists think about precursor isolation in tandem MS.Traditional MS/MS workflows rely on quadrupole filtering to isolate precursor ions prior to fragmentation. But because quadrupoles operate as mass filters, they routinely discard the vast majority of incoming ions — often more than 99% — contributing to signal loss, slower acquisition speeds, and chimeric spectra in complex mixtures.Daniel explains how Structures for Lossless Ion Manipulations (SLIM) technology introduces an additional gas-phase separation step between LC and MS — enabling:* Near-lossless ion transmission through the instrument* Separation based on size-to-charge rather than mass-to-charge* Cleaner MS/MS spectra with reduced spectral chimerism* LC gradient compression without sacrificing analytical resolution* Peak capacities comparable to 20–30 minute LC separations — achieved in milliseconds For chromatographers, this raises an important question:If critical separations can occur in the mobility domain, how much chromatography do we actually need?Daniel also discusses:* Whether HRIM could supplement or replace quadrupoles in future instruments* Applications in proteomics, metabolomics, and environmental analysis* Integrating ion mobility into triple quadrupole workflows* Challenges around method development and data processing* What the next generation of LC-ion mobility-MS platforms may look likeThank you  @SeparationScience  for collaborating with me on this episode!---🎧 Guest: Daniel DeBord, CTO, MOBILion Systems🎙 Podcast: Concentrating on Chromatography📌 Episode 50